Mouse neuroblastoma cell-based model and the effect of epileptic events on calcium oscillations and neural spikes

Suhwan Kim; Unsang Jung; Juyoung Baek; Sangwon Lee; Woonggyu Jung; Jeehyun Kim; Shinwon Kang

doi:10.1117/1.JNP.7.073794

5 November 2013 Mouse neuroblastoma cell-based model and the effect of epileptic events on calcium oscillations and neural spikes

Suhwan Kim, Unsang Jung, Juyoung Baek, Sangwon Lee, Woonggyu Jung, Jeehyun Kim, Shinwon Kang

Author Affiliations +

Journal of Nanophotonics, Vol. 7, Issue 1, 073794 (November 2013). https://doi.org/10.1117/1.JNP.7.073794

Abstract

Recently, mouse neuroblastoma cells have been considered as an attractive model for the study of human neurological and prion diseases, and they have been intensively used as a model system in different areas. For example, the differentiation of neuro2a (N2A) cells, receptor-mediated ion current, and glutamate-induced physiological responses have been actively investigated with these cells. These mouse neuroblastoma N2A cells are of interest because they grow faster than other cells of neural origin and have a number of other advantages. The calcium oscillations and neural spikes of mouse neuroblastoma N2A cells in epileptic conditions are evaluated. Based on our observations of neural spikes in these cells with our proposed imaging modality, we reported that they can be an important model in epileptic activity studies. We concluded that mouse neuroblastoma N2A cells produce epileptic spikes in vitro in the same way as those produced by neurons or astrocytes. This evidence suggests that increased levels of neurotransmitter release due to the enhancement of free calcium from 4-aminopyridine causes the mouse neuroblastoma N2A cells to produce epileptic spikes and calcium oscillations.

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Suhwan Kim, Unsang Jung, Juyoung Baek, Sangwon Lee, Woonggyu Jung, Jeehyun Kim, and Shinwon Kang "Mouse neuroblastoma cell-based model and the effect of epileptic events on calcium oscillations and neural spikes," Journal of Nanophotonics 7(1), 073794 (5 November 2013). https://doi.org/10.1117/1.JNP.7.073794

Published: 5 November 2013

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