Single-cell imaging tools for brain energy metabolism: a review

Abstract. Neurophotonics comes to light at a time in which advances in microscopy and improved calcium reporters are paving the way toward high-resolution functional mapping of the brain. This review relates to a parallel revolution in metabolism. We argue that metabolism needs to be approached both in vitro and in vivo, and that it does not just exist as a low-level platform but is also a relevant player in information processing. In recent years, genetically encoded fluorescent nanosensors have been introduced to measure glucose, glutamate, ATP, NADH, lactate, and pyruvate in mammalian cells. Reporting relative metabolite levels, absolute concentrations, and metabolic fluxes, these sensors are instrumental for the discovery of new molecular mechanisms. Sensors continue to be developed, which together with a continued improvement in protein expression strategies and new imaging technologies, herald an exciting era of high-resolution characterization of metabolism in the brain and other organs.


Metabolism and Neuroscience
The metabolic core of a neuron is similar to that of any other cell, so metabolism is seen as a platform for the unique functions of the brain, with emergent processes such as synaptic transmission, memory, and cognition.According to this view, the brain needs metabolism in the same manner a concerto requires instruments.Even so, it is not the quality of the instruments that makes a concerto unique, but the score or the high-level design, metabolism is not perceived as a major part of what makes the brain unique.
However, the musical analogy does not consider that nervous cells have continued to evolve throughout the process of encephalization, fostered by the allopatric influence of the blood-brain barrier.While the same bits and pieces are being used, the brain has developed its own set of metabolic rules.For example, glutamate, a humble metabolite elsewhere, doubles in the brain as an excitatory neurotransmitter.How are these roles reconciled?Another novelty was the advent of glia, bringing division of labor and a new modulatory level for information processing.5][6][7] Thus, lactate is now regarded as both a fuel and a bona fide signaling molecule.The mismatch between glycolysis and mitochondrial oxidation that determines net lactate production is observed throughout the brain during childhood but becomes restricted in the adult brain to areas expressing neotenous genes associated with synaptic remodeling. 8These tonically active, lactate-rich areas, collectively known as the default mode network, 9 are most vulnerable to amyloid deposition. 10,11Other evidence for a higher role for metabolism relates to energy.Whereas brain tissue is expensive, consuming fuel 10 times faster than the rest of the body, the extraordinary growth of the brain during mammalian evolution took place in a context of chronic food shortage. 12Therefore, evolutionary strategies built around energy constraints are expected to be part of the brain's blueprint.The above examples suggest that metabolism exists not just as a low-level platform, but as a relevant player in information processing.Metabolism is also interesting for practical reasons because the study of cognition in health and disease relies heavily on fluorodeoxyglucose-positron emission tomography (FDG-PET) scanning and functional magnetic resonance imaging (fMRI), techniques that monitor local energy metabolism.Moreover, defective metabolism can be troublesome, and a promising line of research on neurodegeneration has been inspired by the finding of metabolic alterations decades before the onset of clinical symptoms. 10,11eaker, long-range forces that determine the structure of cells and tissues.Thus, microscopic properties of enzymes, such as substrate specificity, withstanding harsh extraction and purification procedures, are what proved fundamental for the mapping of the metabolic network during the first half of the 20th century.At the next level of organization, enzymes associate with other proteins and lipids, interactions that are important for regulation.Except for the inner workings of mitochondria, which are readily purified and studied by respirometry, the subcellular level of metabolic organization is virtually uncharted territory.For instance, there is evidence of physical interaction and functional cross-talk between the glycolytic machinery and the Na þ ∕K þ ATPase, the great energy spender, but the nature of the interaction does not seem to be energetic. 14What are the mechanisms that match energy production to energy consumption?The enzymes of glycolysis are expressed in stoichiometric proportions. 15Is this finding related to their possible association in functional modules?Hexokinase, the first enzyme of glycolysis, is bound to mitochondria and also to the Na þ ∕glutamate co-transporter. 16,17Are these interactions part of the machinery matching glycolysis to mitochondrial respiration?9][20] What are the relative roles of energy status, redox status, substrate supply, and calcium-signaling in the control of mitochondrial flux?Are mitochondria fueled by pyruvate or by lactate? 21How can glycogen be mobilized without generating a traffic jam at phosphofructokinase?These are the kinds of questions that need to be tackled at the subcellular level.
Metabolic organization at the cellular level presents further unresolved issues.In culture and in acute brain slices, astrocytes are more glycolytic than neurons. 22,230][31][32][33] Cultured cells, which provide optimum control of experimental variables, are still an indispensable tool for the discovery of subcellular and cellular mechanisms. 34But the parameter space is more restricted in situ than in culture, and new approaches that are able to probe metabolism at cellular resolution, in tissue slices and in living animals, are required to gauge the pertinence of the molecular mechanisms that are being discovered.There are important questions that need to be addressed through in vivo experiments, such as the concentrations of glucose and lactate in astrocytes, neurons, and oligodendrocytes, the extent and direction of glucose, lactate and pyruvate fluxes within and between cells, how the brain tissue copes with the large changes in local energy demand that accompany neural activity, the metabolism of subtypes of cells, and the metabolism of the diseased brain.

High-Resolution Imaging of Metabolism
Metabolic processes span many orders of spatiotemporal magnitude, from submillisecond nanometer enzyme catalysis to the years that it takes to deposit an amyloid plaque.One technique that may cover the full range of metabolism is fluorescence microscopy.Wide-field, confocal, multiphoton, light-sheet, super-resolution and other microscope systems need to be combined with a toolkit of fluorescent probes to make metabolism visible in the way calcium dyes do for neuronal activity.So far metabolism has been imaged using NAD(P)H and flavoprotein autofluorescence, fluorescently labeled metabolites, and, more recently, with genetically encoded nanosensors.6][37][38][39] The extremely rapid response of NADH is consistent with its turnover time, which in brain cells is in the order of a few milliseconds. 40However, the interpretation of autofluorescence data is limited by several factors, including uncertainty about the relative weight of free and protein-bound NADH, interference from NADPH, which in the cytosol is much more abundant, difficulties in assigning the signals to specific subcellular compartments, and the inherent ambiguity of concentration with respect to flux, compounded by the requirement for UV light, which is toxic and unavailable in standard confocal microscopes.
An alternative approach is the use of fluorescently tagged metabolites, 41 as was done with glucose and its fluorescent analogues.They provide good signal-to-noise ratio, but because the tag interferes with the biochemical function of the metabolite, these compounds may only be used as tracers.For example, in the fluorescent glucose analogs 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxy-glucose (2-NBD-glucose) and 6-NBD-glucose, the fluorescent group is hydrophobic, which enhances binding to the glucose transporter, severely slowing down membrane translocation. 42In this particular case, the slowness of uptake is an advantage, as it provides an extended time window for experimental intervention.With these tools, it has been shown that astrocytic glucose transport is rapidly

Inter-modular interactions (weak)
Intra-modular interactions (strong) Fig. 1 Organization of metabolism.The living organism is represented as a hierarchical stack of modular systems.Interactions at any level are stronger than at higher levels, which is the basis for dissectability.In this example regarding metabolism, the enzyme hexokinase (represented by a blue oval) is shown to be constituted by amino acids (black ovals).In the next level up, hexokinase associates with other enzymes and cofactors to constitute the glycolytic machinery (green oval).Further up the organizational ladder, the glycolytic machinery associates with organelles and other structures to form an astrocyte (yellow oval), which in turn interacts with neurons and vessels to shape the neurogliovascular unit (orange oval) [after J. G. Miller's generalized living system 13 ].
stimulated in an activity-dependent fashion in vitro and in vivo [43][44][45][46] and that metabolite flux through gap junctions may sustain synaptic activity. 47Once inside cells, 2-NBDG is slowly phosphorylated by hexokinase and trapped as 2-NBDG-P, a readout of the glycolytic rate that becomes accessible after free tracer wash-out.Application of 2-and 6-NBDG protocols to acute slices prepared from cerebellum and hippocampus showed that astrocytes and Bergmann glia are several-fold faster at transporting and metabolizing glucose than neurons, 23,48 consistent with constitutive lactate shuttling from glia to neurons. 49,50As its accumulation is limited by transport and not by metabolism, 2-NBDG uptake does not provide sufficient temporal resolution for investigation of the rapid events underlying fMRI or the rapid fluctuations detected by real-time measurement of tissue autofluorescence, oxygen, and lactate.The most recent addition to the metabolic imaging toolkit is the genetically encoded nanosensor.Typically, a nanosensor is a fusion protein comprising a bacterial protein that binds a specific metabolite, and one or two fluorescent proteins.Binding of the metabolite to the bacterial moiety leads to a conformational change that modifies the physical properties of the fluorescent partner(s).Some sensors have two fluorescent proteins with overlapping emission and excitation spectra, capable of Note: There are six metabolites for which there are optical nanosensors available that have been validated in mammalian cells.The position of these molecules in the metabolic network is indicated in Fig. 3. a In vitro measurement of purified protein (data not available for the sensor expressed in cells).
Addgene is a nonprofit plasmid repository (www.addgene.org) undergoing Förster resonance energy transfer (FRET), and they respond to the metabolite with a change in FRET efficiency.Being ratiometric, FRET measurements are insensitive to sensor concentration, volume changes, and small focal drifts.Other sensors have only one fluorescent protein, which responds to metabolite binding with a change in emission intensity.Single fluorophore sensors are not intrinsically ratiometric, but a ratio may be obtained by coexpression of a second fluorescent protein.Since the introduction of Cameleon, the first FRET nanosensor in 1997, 51 many probes have been made available to measure ions, enzyme activities, and metabolites in different cell systems from bacteria to whole animals.
Herein we review a restricted set of nanosensors for the six energy metabolites that have so far been validated in mammalian cells: glucose, glutamate, ATP, NADH, lactate, and pyruvate (Table 1).The ligand-binding moiety of these nanosensors is either a periplasmic protein involved in signaling or a transcriptional factor.The first metabolite nanosensors were built with cyan fluorescent protein (CFP) and yellow fluorescent protein (YFP) as an FRET pair, and displayed a small change in fluorescence ratio upon ligand binding (<10%).were exposed to the mitochondrial blocker sodium azide (5 mM).Although the metabolic depletion in one of them appears to be stronger, this may not be the case (see text).(b) An astrocyte expressing Peredox 118 in the cytosol was exposed to a rise in extracellular K þ from 3 to 12 mM.The observed increase in NADH∕NAD þ ratio is consistent with a primary stimulation of glycolytic NADH production, but not with a stimulation of mitochondrial NADH consumption.(c) The uptake of 5 mM lactate was measured in a T98G glioma cell using Laconic Much as calcium transients reveal cellular activity, the most frequent use of metabolite nanosensors is for the detection of changes in metabolism.For example, the depletion of glucose observed upon removal of extracellular glucose, 52 the release of glutamate by synaptic activity, 83 and the depletion of ATP that follows mitochondrial inhibition. 91A change in metabolite level during a given experimental challenge shows that metabolism has been altered, but in contrast to calcium transients, the interpretation of metabolite fluctuations may be ambiguous.For instance, Fig. 2(a) illustrates an experiment in astrocytes expressing ATeam, a FRET nanosensor for ATP.Two behaviors were observed in response to oxidative phosphorylation (OXPHOS) inhibition: one cell showed a strong decrease in fluorescence ratio, while another cell in the same field was less affected.This difference could be explained by different changes in ATP concentration, but could also be due to a different resting ATP level.Moreover, even if the resting ATP levels were similar, it is not possible to tell whether the different response is due to (1) mitochondrial function, (2) the capacity of glycolysis to compensate for the loss of ATP production, or (3) perhaps ATP expenditure.Relative levels may be more informative if some complementary information is available.
We have reported previously that astrocytes acutely increase their rate of glucose consumption in response to elevated extracellular K þ . 31,59In principle, the stimulation may be a "pull" phenomenon, secondary to mitochondrial consumption of cytosolic NADH, as hypothetized for Ca 2þ -dependent stimulation in neurons, 18,20 or alternatively, it may be a "push" phenomenon, in which regulation acts on the glycolytic machinery.The NADH∕NAD þ nanosensor Peredox provides an answer to this question by showing that K þ -stimulated astrocytes acutely increase their cytosolic NADH∕NAD þ ratio [Fig.2(b)], thus evidencing that the primary target of K þ is not mitochondrial NADH consumption but the glycolytic machinery.Of note, Peredox provides a better readout than NAD(P)H autofluorescence in terms of specificity and signal-to-noise ratio; it is less phototoxic and offers the possibility of targeted expression, but its temporal resolution is lower (seconds versus milliseconds) and contributes to NADH buffering.It should be borne in mind that the rate at which a given metabolite departs from its steady-state level depends not only on the degree of perturbation but also on the turnover time of the metabolite, i.e., the ratio between concentration and flux through the pathway, which may differ by orders of magnitude along a given pathway.A detailed analysis of this important point and its consequences may be found in Ref. 78.Another use of uncalibrated probes is for the study of membrane transporters.For example, the experiment in Fig. 2(c) shows a glioma cell expressing the lactate sensor Laconic that was sequentially exposed to lactate alone or in the presence of the monocarboxylate transporter (MCT) blockers phloretin or p-chloromercuriphenylsulfonic acid (pCMBS).If rates are compared at similar FRET ratios, it is possible to determine degrees of inhibition, even if the actual concentrations are unknown.Determination of absolute concentrations can be performed by calibrating the sensor, ideally in the same cell type in which measurements are being made.Full calibration (i.e., determination of a dose-response curve in situ) has been achieved only for the glucose sensor, by blocking glucose consumption while using the glucose transporter or an artificial pore to equilibrate glucose across the plasma membrane. 56,62Detailed protocols have been made available for the use of metabolite sensors in mammalian cells 54 and more specifically for the use of the glucose sensor. 78It is not feasible to halt the metabolism of pyruvate, lactate, or NADH without compromising cell viability, but the corresponding sensors have been two-point calibrated by obtaining readouts at extreme values of the parameters in combination with the affinity constants measured in vitro. 118,121,122Thanks to calibration, a glucose gradient was detected across the astrocytic plasma membrane, 56,62 consistent with a modulatory role for GLUT transporters, and astrocytes were found to have higher NADH∕NAD þ ratios than neurons, 118 supporting the shuttling of redox equivalents between astrocytes and neurons. 123Even over a single microscopic field, large differences were detected in glucose concentration and glycolytic flux in adjacent cells, the kind of diversity that may be mined by systems biology approaches, both to extract mechanistic information 124 or to serve as readouts for single-cell metabolomics. 125No calibration is possible for the ATP sensors, so they are considered semiquantitative.A more refined use of the sensors is for the determination of flux through specific pathways.For this purpose, the probe needs to be calibrated, so that the fluorescence ratio is converted into absolute concentration.The strategy for flux determination is to halt flux at a known point in the pathway and then to monitor the rate of accumulation of an upstream metabolite or the rate of depletion of a downstream metabolite.With this approach, it has been possible to estimate the rate of glucose consumption (FLIP 12 glu700μΔ6), the rates of lactate production or consumption (Laconic), and the rate of mitochondrial pyruvate consumption (Pyronic), as illustrated in Fig. 2(d).Some biological phenomena that have been identified with flux protocols are the regulation of astrocytic glycolysis by high K þ and glutamate, 31,59 feedback control of glycolysis by lactate, 65 mitochondrial flux in electrically stimulated neurons, 122 and the Warburg effect in T98G glioma cells. 121sing the FRET nanosensors in vivo, Bruno Weber and colleagues have successfully imaged glucose, lactate, and pyruvate in astrocytes and neurons using two-photon microscopy.Practical issues to be addressed for in vivo studies include sensor expression levels, calibration, the metabolic effects of anesthesia, and the adequacy of "inhibitor-stop" protocols for flux determination.
On a cautionary tone, it is important to keep in mind that each nanosensor has its own limitations.A common one affecting selectivity is sensitivity to pH, which may be corrected for by performing parallel measurements with pH-sensitive dyes.Another one is possible interference with cellular functions.With current high-end detectors, it is necessary to express ∼10 μM of the nanosensor to obtain a good signal-to-noise ratio. 126The cytosolic concentrations of glucose, glutamate, ATP, lactate, and pyruvate are all much higher, so nanosensor buffering is not a concern for these molecules.NADH is in the nanomolar range 120 and, therefore, the sensor contributes to the NADH buffering capacity.These and other limitations of the sensors like phototoxicity, toxicity of the fluorescent proteins, and difficulties for subcellular targeting are analyzed elsewhere. 78,127,128Concluding Remarks Metabolism makes up a large part of what cells are, the sum of a finite number of chemical reactions involving interactions between structural and functional modules at different levels of complexity.In the brain, metabolism is also a player in high-order processes, such as synaptic remodeling, memory, and cognition.In order to tackle the metabolic modules and the interactions between them with a reductionist or systems biology approach, it is necessary to develop new technologies.Genetically encoded nanosensors are noninvasive and can be used from cultured cells to living animals, spanning several metabolic hierarchies.Nanosensors for six metabolites have been validated in mammalian cells, which allow real-time monitoring of relative level and, in some cases, of concentration and flux.A comprehensive study of the metabolic network will require the development of new sensors, most urgently for metabolites involved in glycogen cycling, the pentose phosphate pathway, and the Krebs cycle.Together with the continued improvement in protein expression strategies and new imaging technologies, metabolite nanosensors herald an exciting era of high-resolution characterization of metabolism in the brain and other organs.The relative location within the metabolic network of the six metabolites covered in this review gives some idea of how much remains to be done (Fig. 3).

Fig. 2
Fig. 2 Examples of the use of genetically encoded nanosensors for the estimation of metabolite levels, concentrations, and fluxes in single cells.(a) Two adjacent astrocytes expressing ATeam 1.0391 were exposed to the mitochondrial blocker sodium azide (5 mM).Although the metabolic depletion in one of them appears to be stronger, this may not be the case (see text).(b) An astrocyte expressing Peredox 118 in the cytosol was exposed to a rise in extracellular K þ from 3 to 12 mM.The observed increase in NADH∕NAD þ ratio is consistent with a primary stimulation of glycolytic NADH production, but not with a stimulation of mitochondrial NADH consumption.(c) The uptake of 5 mM lactate was measured in a T98G glioma cell using Laconic 121 in the absence or presence of the MCT blockers phloretin (50 μM) and pCMBS (500 μM).The partial inhibitory effect of phloretin becomes evident.(d) Determination of metabolic fluxes with inhibitor-stop protocols.In the steady state, the cytosolic concentrations of glucose, lactate, and pyruvate are constant.Interruption of the steady state with a blocker of the glucose transporter GLUT (cytochalasin B) or of the monocarboxylate transporter MCT (AR-C155858) produces depletion or accumulation at an initial rate equal to the steady-state flux of the pathway.The right panels provide examples of astrocytic glucose consumption estimated with 20 μM cytochalasin B, 56 HEK293 lactate production estimated with 1 μM AR-C155858,122 and astrocytic pyruvate consumption estimated in the absence of glucose and lactate with 1 μM AR-C155858, as detailed in Ref.122.Rates are indicated.

Fig. 3
Fig. 3 Mammalian metabolic networks.Schematic representation of mammalian metabolism.Points correspond to metabolites and lines to chemical transformations (from Alberts et al., 1983, cited in Ref. 129).The network location of the six metabolites that have been imaged in mammalian cells is indicated: glucose (glc), pyruvate (pyr), lactate (lac), glutamate (glu), NADH, and ATP.

Table 1
Genetically encoded sensors for molecules involved on brain energy metabolism.
122 Determination of metabolic fluxes with inhibitor-stop protocols.In the steady state, the cytosolic concentrations of glucose, lactate, and pyruvate are constant.Interruption of the steady state with a blocker of the glucose transporter GLUT (cytochalasin B) or of the monocarboxylate transporter MCT (AR-C155858) produces depletion or accumulation at an initial rate equal to the steady-state flux of the pathway.The right panels provide examples of astrocytic glucose consumption estimated with 20 μM cytochalasin B,56HEK293 lactate production estimated with 1 μM AR-C155858,122and astrocytic pyruvate consumption estimated in the absence of glucose and lactate with 1 μM AR-C155858, as detailed in Ref.122.Rates are indicated.Neurophotonics 011004-4 Jul-Sep 2014 • Vol.1(1) San Martín et al.: Single-cell imaging tools for brain energy metabolism: a review Downloaded From: https://www.spiedigitallibrary.org/journals/Neurophotonics on 16 Sep 2023 Terms of Use: https://www.spiedigitallibrary.org/terms-of-use 4 Some Applications of the Sensors: Relative Level, Concentration, and Flux