Dynamic calcium and voltage imaging has been a major tool in cellular neuroscience for over 40 years.1,2 In that time, there have been major improvements in indicators, microscopes, imaging systems, and computers. Cutting edge research has trended toward genetically encoded calcium indicators (GECIs) or voltage indicators (GEVIs) because of their clear advantage in targeting specific cell types, and use of two-photon microscopes, and in vivo preparations. However, it is worth noting that some questions still may be best approached using more classical methodologies. For example, although recently developed GECIs3,4 and GEVIs56.7.–8 have greatly improved sensitivity and kinetics compared with the early generation of these probes, and are beginning to be used in physiologically relevant studies,9 they are still not as sensitive or as fast as some of the popular organic indicators like Oregon Green BAPTA-1 (OGB-1), OGB-5N, and fluo-4 (for calcium) or RH-795, di-4-ANEPPS, and di-2-ANEPEQ (for voltage).
Similarly, two-photon microscopes, light sheet microscopes, and other confocal systems have clear advantages in thick tissue where optical sectioning is important and where the cells of interest may be hundreds of microns below the surface of the preparation. However, if these considerations are not critical, then some modern charge-coupled device (CCD) cameras may offer a combination of speed, sensitivity, and field of view that scanning systems cannot achieve.
In this review, we highlight a few examples of experiments where high-speed CCD imaging and classical indicators have revealed information that has so far been more informative than results using genetically encoded indicators and two-photon systems, and, in some cases, has not even been detected by these newer systems. The Cohen laboratory has been a primary force in developing and exploiting this approach.1,2,10,11 The newer results described below build on these pioneering experiments.
[Ca2+]i Measurements in Slices and Culture
One interesting example is the measurement and analysis of calcium release from internal stores. These increases occur in the form of waves, sparks, and puffs whose properties cannot be easily inferred from electrical measurements.12 In particular, the puffs and sparks occur at unpredictable locations, making it necessary to look over an extended region to enable the detection (Fig. 1). These events are often very fast (rise times less than 10 ms, fall times less than 70 ms) requiring a high-speed detection system and fast responding indicators, especially if the measurement of diffusion from the spark source is important. If a neuron in a slice is filled with an organic indicator from a patch electrode and the cell (or part of the cell) is not too far below the surface of the slice, then some of the advantages of two-photon systems are not critical. For example, sections of thin dendrites and axons can be examined using an ordinary microscope and a CCD camera since the neuronal processes are thinner than the depth of focus of either system. In these conditions, one advantage of a CCD detector system is that the high-speed images can be made from spatially extended structures while detection at similar speeds with a two-photon system requires that the measurements use a line scan mode, restricting signals to very small areas or straight segments. This property has been useful in the detection and measurement of sparks and puffs in dendrites13,14 and in total internal reflection fluorescence measurements in cultured neuroblastoma SH-SY5Y cells.15 A line scan across the dendrite cannot be positioned at the right location to detect a spark since that location is not known in advance. Even if that location was known, a transverse line scan would reveal neither the spatial extent of the spark or puff nor the dynamics at different locations. In principle, a scan along a dendritic segment might capture a spark and its spatial extent, but such measurements have not been reported. Video rate confocal systems have been used to follow changes in dendrites16,17 but did not detect sparks or puffs, possibly because the frame rate and sensitivity were not adequate. These considerations would not apply to the detection of a change due to an action potential that backpropagated over the dendrites [backpropagating action potential (bAP)] since these signals are almost the same at most locations and, therefore, a transverse line scan is almost certain to detect this signal and to reveal the important physiological information (when and if the spike occurred and the duration of the change). One group18 did use two-photon microscopy to detect spark-like events in presynaptic terminals, imaging small regions in the terminal at 100-ms frame interval. Detection with this slow rate was successful in part because the sparks were so long-lasting in the terminal (over 1 s, possibly because a high concentration of indicator was used, which would severely buffer and slow the transients).
Two-photon microscopes and other confocal systems have been used to detect sparks in myocytes19 and muscle fibers20 by aligning the scan along the axis of the cell. Line scans along the fiber axis at 500 Hz were able to detect the very rapid (4-ms rise time, 9- to 15-ms fall time) sparks in muscle cells but may have missed some events since myocytes are wider than the scan width. Line scans in oocytes21 detected some -mediated puffs below the surface of the cell, but could not determine where the scans intersected the mass of the change and, therefore, could not accurately determine the kinetics and spatial extent of these localized events.
So far, these puffs and sparks have only been detected with organic calcium indicators, whether the detector was a CCD camera or a two-photon system. There are several reasons why these indicators may be preferred for this application compared with GECIs apart from the fact that the GECIs are newer and have not been widely tested. First, the signals are usually small in neurons, typically 20% to 80% of the amplitude of a bAP signal at the same location in the apical dendrites of a pyramidal neuron.22 Single spike-evoked signals detected with GECIs are often close to the noise level in many neurons, suggesting that the spark and puff signals would be even harder to detect. Second, the spark signals come from localized regions, making it difficult to spatially average the signal to improve the S/N. In contrast, spatial averaging of the spike signal over the entire somatic region often has been used, especially in in vivo experiments. Third, the spark fluorescence signals are usually faster than spike-evoked signals because the decay time of the spark transient is shortened by diffusion away from the localized source in addition to removal by a pump. Since the kinetics of GECIs are slower than those of organic indicators, the intrinsic speed of the underlying transient will lead to filtering of the transients making them harder to detect. A related issue is buffering of the transients by the indicator. It is well known that buffering by exogenous indicators slows the fluorescence transients and reduces their amplitude.23,24 Because the concentration of organic indicator is known (at equilibrium equal to the concentration in the pipette), the effect of this buffering can be calculated and extrapolated to a zero indicator concentration. In contrast, the concentration of GECIs in cells is not known and their buffering effect has not been determined or corrected for in current experiments. Nevertheless, recent developments with GECIs have shown that they can detect signals from small volumes like spines4 and can detect fast spike-evoked signals,25 so it may be possible in the future for GECIs to be used in spark detection and analysis.
Much less attention has been addressed to follow changes in neurons. Only a few indicators for this ion have been developed and the best one, sodium-binding benzofuran isophthalate (SBFI), was synthesized over 30 years ago.26 No genetically encoded indicators for this ion have been developed. All the reported experiments were done in slices or in culture. There are no in vivo studies, probably because the signal size is usually much smaller than with calcium indicators and, in most cases, the goal of the experiments, to optically detect spikes in neurons, can be accomplished easier with calcium measurements. Nevertheless, several investigators reported interesting measurements in slices using CCD cameras.27,28 Very high speeds were usually not necessary for these early experiments; video rates were adequate to detect the signals (Fig. 2). Since the signal size was small, it was important to optimize all the components in the systems and to use cameras with good quantum efficiency and low noise. In several cases, an important part of the experiment was to detect and interpret the diffusion of sodium away from a localized source.27,2930.–31 Since diffuses about 100 times faster than free in cytoplasm, the physiological consequences of diffusion are different for these two ions. In these experiments, high camera frame rates were important; in some cases, rates of up to 500 Hz were useful (Fig. 3). A few experiments measured changes in dendrites and spines with a two-photon microscope using line scans to achieve high-time resolution.32,33 But these measurements, while informative, did not detect the signals with the same S/N as with CCD cameras and could not resolve synaptically activated subthreshold changes.
Voltage Sensitive Dye Imaging
Understanding the function of individual neurons requires that we measure, analyze, and understand spatiotemporal dynamics of electrical signals. Electrical signals are measured directly and most precisely using electrodes. However, the electrode measurements report voltage changes from a single location on a neuron, while the electrical behavior of other parts of the cell remains uncertain. One can extend intracellular electrode measurements to a few locations but never to tens or hundreds of locations. A second limitation is that small structures like axons and axon collaterals, terminal dendritic branches, and dendritic spines do not tolerate electrodes and, as a rule, cannot be probed for electrical signals. Voltage sensitive dye (VSD) recording techniques (-imaging) can solve both these problems, allowing multiple site recordings of electrical events from all locations on individual neurons. However, to be effective, these kinds of measurements must use indicators that accurately follow the voltage profile at the recording site and must use a measuring system that has the temporal and spatial resolutions to distinguish important differences in the signals from different locations.
In the first example, high-speed -imaging with organic VSDs and a NeuroCCD-SMQ camera34 was utilized to monitor action potential signals from axons in brain slices. The measurements were carried out to determine the location and length of the spike trigger zone and to analyze the pattern of spike initiation and propagation in the axon of layer 5 cortical pyramidal neurons3536.–37 and Purkinje cells of the cerebellum.38,39 The type of raw optical recordings and the data analysis procedures utilized in these experiments are illustrated in Fig. 4. From this kind of measurement, it is possible to determine the location and length of the axonal spike initiation site. These two parameters are obtained directly from multisite optical recording of the membrane potential transients either by investigating spike latencies at the soma/axon hillock and more distal axonal recording locations or by the inspection of the spatial distribution of membrane potential as a function of time. These measurements used the NeuroCCD-SMQ camera operating at 10 kHz to achieve the required high-time resolution. In previous experiments,40 it was established that the electrochromic VSDs track the voltage signals practically instantaneously at the biological time scale (time constant faster than . The ability to monitor the spike initiation in the axon initial segment is important because it has been recently discovered that intricate details in the spatial pattern of channel clustering play a critical role in tuning neuronal computations and changes in channel distribution have been shown to mediate novel forms of neuronal plasticity in the axon.41
In the second example, high-speed -imaging with a CCD camera in wide-field epi-fluorescence microscopy mode was utilized to re-examine the specific question of the transfer of dendritic electrical signals across spine neck to spine heads. Using the camera enabled the detection from the spine head and nearby dendritic regions simultaneously.34 The sensitivity and the spatiotemporal resolution of -imaging were adequate to simultaneously determine the amplitude and the time course of bAP signals in the heads of individual spines and in parent dendrites (Fig. 5). These measurements were carried out in four groups of spines with different neck lengths. The recordings of membrane potential transients from individual spines linked to a computational model provided evidence that synapses on spine heads are not electrically isolated from membrane potential signals in the parent dendrite.42 It is possible to use two-photon detection of VSD signals from dendritic spines.43 However, to make these measurements at sufficient speed (over 10 kHz) to follow the voltage transients, the beam could not move and measurements were reported from only a single voxel.
Conclusions and Future Prospects
The diversity of currently available calcium and voltage indicators provides opportunities to do many different kinds of experiments. At the time when only organic indicators were available, the most significant properties that mattered to users were sensitivity, i.e., how good was the S/N for detecting the event of interest, and the kinetics, which reflected how well the indicator followed the time course of the underlying event. Other important properties include the wavelength of excitation and emission and how easy it was to load the indicator into the cell or to stain the preparation. The wavelengths were important because some indicators were used in cells that had light sensitivity and it was useful to excite outside this sensitive region. Indicators with excitation bands at longer wavelengths were also less likely to cause photodynamic damage. In addition, some indicators were used in experiments where other signals were examined and it was useful to have no wavelength overlap in the signals. For calcium indicators, it was also important in some experiments to have enough sensitivity to permit the indicator to be used at a low enough concentration so as not to be a significant buffer. For VSDs, it was important for the indicators to accurately track the voltage change, to have good sensitivity, to have few pharmacological effects, and to cause the minimal photodynamic damage. Many different voltage indicators, sensitive in different wavelength regions, are currently available. For sodium indicators, the available probes are not as diverse. The original probe, SBFI,26 is still the one used in most experiments, although some new indicators have just become available.44 Probes are also available to report on the changes in chloride concentration and pH, but these are not extensively used for examining real-time neuronal signaling.
Genetically encoded indicators potentially offer the additional important properties that they can be targeted to specific cell types and to specific parts of a cell, and they can be encoded into the genome, and be transmitted through generations and available for long-term studies. They may also be less toxic to the neurons in which they are inserted, but this property has yet to be examined rigorously. However, the kinetics, linearity, and sensitivity of currently available probes are not yet as good as many organic indicators and this limits the kinds of measurements that can be made. An additional factor is that these probes are not easily titrated in cells; if the concentration is not known, then the buffering or other effects of the indicator cannot be controlled.
So far, the main use of GECIs and GEVIs has been to detect action potentials or other measures of electrical activity in the brain. For these applications, the main requirements are sensitivity and sufficiently rapid kinetics to distinguish individual action potentials. Although there is significant progress in this field,4,25 many currently available GECIs do not respond rapidly enough to separate the spikes in a burst, so spike numbers often have been inferred from the amplitude of the signal. This approach is not always accurate. Also, the kinetics are still too slow to accurately follow the removal of calcium in cells or the rise time of the changes in those neuronal compartments where they have been evaluated. Similarly, GEVIs, while far from optimized, have best been used to detect in vivo neuronal activity and subthreshold events in Drosophila.9 Progress in this field is rapid. An early version of ArcLight had kinetics fast enough to resolve individual spikes in bursts at 30 Hz,5 while an even newer GEVIs like ASAP1 and ArcLight A173 can follow signals at over 100 Hz.67.–8 Nevertheless, these probes are still too slow to track the details of spike shape or the propagation of action potentials within individual cells.
It is hard to predict the future of imaging systems since developments are occurring in many different directions. In this review, we have concentrated on the ways in which high-performance CCD cameras can reveal information that is difficult to acquire using two-photon microscopes, while recognizing, of course, the many advantages of multiphoton systems. One advantage of CCD systems is that they can examine spatially extended regions at high frame rates (10 kHz in the examples above). Three groups4546.–47 have developed programmable multisite sampling methods for two-photon imaging that might have the speed to detect the propagation along axons or dendrites. These systems use acousto-optical deflectors to position the laser spot to select a number of arbitrary sites for repetitive sampling at 500 Hz to several thousand hertz. These systems can be considered as line scan detectors with arbitrary paths and point separations. So far, they have only been used to measure the spike generation and propagation using calcium indicators. They have not been used to make VSD measurements or to detect and analyze sparks. It is not clear if this kind of sampling system can achieve the S/N that the high-speed VSD recordings require. CCD cameras, which are constantly detecting light at all locations, may be better, especially if signals can be spatially averaged. Another potential limitation is that in this approach, the investigator has to select the sampled points in advance and so might miss critical events at other locations if they are localized and cannot be predicted before the trial. One limitation with both CCD cameras and two-photon systems is photodynamic damage and indicator bleaching. The higher the frame rate and the smaller the sampled voxel, the higher the light intensity required to make a measurement with an adequate S/N, leading to greater damage. This problem has been apparent almost since the beginning of dynamic imaging experiments over 40 years ago. While not all indicators generate the same level of damage, suggesting that the screening for better probes might be useful, and several technical schemes for reducing injury have been tried,48,49 this still remains a serious problem.
The work in the author’s laboratories relevant to this review was supported by NIH Grants NS085729, NS016295, NS 068407, and NSF Grant IOS0817969.
William N. Ross is professor of physiology at New York Medical College, where he has been for 35 years. He received his PhD in physics from Columbia University in 1969. He learned neuroscience with Larry Cohen at Yale Medical School and Ann Stuart at Harvard Medical School. His research concerns the properties of dendrites in CNS neurons in brain slices. In this work, he uses and develops techniques for high-speed calcium and sodium imaging.
Kenichi Miyazaki is currently a postdoctoral fellow in the laboratory of Dr. William Ross in New York Medical College. He received his PhD from Tohoku University in Japan in 2006, where he studied presynaptic function at the mossy fiber synapse in the hippocampus using electrophysiological and optical recording techniques. His current work concerns the function of dendrites of CA1 pyramidal cells using calcium and sodium imaging techniques.
Marko A. Popovic is a postdoc in the Nederlands Herseninstituut in Amsterdam. In 2009, he received his PhD in biology from Belgrade University. He has completed postdoctoral training working with Dr. Dejan Zecevic at Yale University, where he learned and advanced functional imaging of neuronal compartments with special focus on the voltage imaging field pioneered by Dr. Larry Cohen. Currently, he is studying axon signaling properties in brain slices, both in control conditions and disease models.
Dejan Zecevic is a senior research scientist at Yale University School of Medicine. He received a PhD degree in biophysics from University of Belgrade, Serbia, and was trained in the laboratory of Dr. Lawrence Cohen. Dejan’s work on electrical and functional structure of individual neurons utilizes intracellular voltage-sensitive dye imaging technique, a unique and a cutting-edge technology for monitoring membrane potential signals in axons, dendrites, and dendritic spines.