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12 October 2015 Differential pathlength factor informs evoked stimulus response in a mouse model of Alzheimer’s disease
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Abstract
Baseline optical properties are typically assumed in calculating the differential pathlength factor (DPF) of mouse brains, a value used in the modified Beer–Lambert law to characterize an evoked stimulus response. We used spatial frequency domain imaging to measure in vivo baseline optical properties in 20-month-old control (n=8) and triple transgenic APP/PS1/tau (3xTg-AD) (n=5) mouse brains. Average μa for control and 3xTg-AD mice was 0.82±0.05 and 0.65±0.05  mm1, respectively, at 460 nm; and 0.71±0.04 and 0.55±0.04  mm1, respectively, at 530 nm. Average μs for control and 3xTg-AD mice was 1.5±0.1 and 1.7±0.1  mm1, respectively, at 460 nm; and 1.3±0.1 and 1.5±0.1  mm1, respectively, at 530 nm. The calculated DPF for control and 3xTg-AD mice was 0.58±0.04 and 0.64±0.04 OD mm, respectively, at 460 nm; and 0.66±0.03 and 0.73±0.05 OD mm, respectively, at 530 nm. In hindpaw stimulation experiments, the hemodynamic increase in brain tissue concentration of oxyhemoglobin was threefold larger and two times longer in the control mice compared to 3xTg-AD mice. Furthermore, the washout of deoxyhemoglobin from increased brain perfusion was seven times larger in controls compared to 3xTg-AD mice (p<0.05).

1.

Introduction

Alzheimer’s disease (AD) is a neurodegenerative disease affecting 35 million people worldwide.1 There is interest in developing in vivo imaging methods to enhance our understanding of the disease and to help facilitate earlier intervention.2,3 Most AD cases (60% to 90%) are associated with ischemic vascular disease, and 90% of AD patients exhibit cerebral amyloid angiopathy (CAA), a vascular disease caused by amyloid deposition in the vessels.1 An association between AD and reduced vascular reactivity, a physiological consequence of CAA, was demonstrated with transcranial Doppler,46 functional magnetic resonance imaging (fMRI),7,8 positron emission tomography (PET),9 and single-photon emission computed tomography (SPECT)10 techniques. Neurovascular coupling (NVC), the localized vasodilation that occurs from a specific metabolic demand, is also reduced in AD with visual11 and verbal fluency12 challenges. Therefore, accurate development of imaging biomarkers sensitive to vascular reactivity and NVC is of great interest for early detection of AD.

Rodent models of AD are useful for studying the timecourse of pathology. In the triple transgenic APP/PS1/tau (3xTg-AD) mouse model13 of AD, our previous work demonstrated significant differences in baseline absorption and scattering properties at near-infrared (NIR) wavelengths (650 to 970 nm) as well as magnitude differences in brain oxygenation after inhaled-hyperoxia.14 The 3xTg-AD mice developed accelerated vascular pathology as toxic amyloid and tau proteins accumulated in the brain.15 Others observed changes in NVC in a CAA mouse model with laser speckle imaging.16 NVC primarily has been studied in rats with multispectral imaging, which relies on the modified Beer–Lambert law (MBLL) [Eq. (1)]17,18 to fit oxy- and deoxyhemoglobin (HbO2 and Hb, respectively) concentration changes, based on reflectance changes at two or more wavelengths of light,

Eq. (1)

ΔA(λ,t)=[E(λ)×Δc(t)×DPF(λ)],
where ΔA(λ,t) is the base-10 logarithm of the normalized change in reflectance at wavelength λ and time t, E(λ) is the wavelength-dependent extinction coefficient of chromophore c, Δc(t) is the change in concentration of chromophore c at time t, and DPF(λ) is the differential pathlength factor defined mathematically as ΔA/δμa. The absorption (μa) and reduced scattering (μs) coefficients used to calculate DPF(λ) are usually assumed a priori to be constant values. However, we demonstrated that the baseline optical properties of AD and control mice are significantly different.14,15,19

Hence, we postulate that the use of assumed baseline optical properties may lead to errors in calculating changes in hemoglobin concentrations, especially when models of cerebrovascular disease are used. The objectives of this study are twofold: (1) to compare hemodynamic response to evoked stimuli in 3xTg-AD and control mice and (2) to compare the evoked responses in mice when using commonly assumed baseline optical properties versus directly measured optical properties in the MBLL calculation. We found the baseline optical properties in both controls and AD mice measured using spatial frequency domain imaging (SFDI) differed from literature-reported optical property values, resulting in significantly different calculations of hemoglobin changes in multispectral imaging of an evoked stimulus response.

2.

Methods

2.1.

Animals

We imaged 20-month-old C57BL/6 mice (n=8) and 3xTg-AD mice (n=5) under 1.2% isoflurane anesthesia in a 21% oxygen/79% nitrogen air mix (Oxydial, Starr Life Sciences Corp.). Isoflurane is a strong vasodilator at higher concentrations (>1.6%),20 but cortical vasodilatory reserve has been shown to be intact in rodents anesthetized under 1.2% isoflurane.15,21 After inducing anesthesia, the head of each mouse was secured in a stereotaxic frame (Stoelting Co.), and body temperature was maintained at 37°C with a thermistor-controlled heating pad (CWE, Inc.). We removed the skin above the scalp and thinned the skull within a 3mm×3mm region of interest (ROI) bilaterally over the hindpaw cortical sensory area with a dental drill. A well was created with petroleum jelly around the ROI, filled with sterile saline and covered with a glass coverslip to maintain optical transparency of the thin skull. All procedures were performed in accordance with the regulations of the Institutional Animal Care and Use Committee of the University of California, Irvine (protocol 2010-2934).

2.2.

Spatial Frequency Domain Imaging

SFDI is a wide-field, camera-based technique capable of measuring absorption (μa) and reduced scattering (μs) coefficients in tissue on a pixel-by-pixel basis. SFDI works by structuring light into two-dimensional sinusoidal patterns and projecting them onto the tissue surface.22 The tissue acts as a spatial filter and blurs the structured patterns. By projecting patterns of differing spatial frequencies, the modulation transfer function of the tissue can be found, from which a unique pair of optical absorption and scattering coefficients is determined for each pixel in the ROI.23

The flexible LED and modulation element (FLaME) platform used for SFDI and multispectral imaging was described previously24 and is shown in Fig. 1. We sequentially projected five spatial frequencies (0, 0.05, 0.1, 0.2, and 0.4mm1), with each sinusoidal frequency pattern projected three times, at phases of 0, 120, and 240 deg. The remitted reflectance was captured by two cameras (460 and 530 nm, 10-nm FWHM bandpass filters, Thorlabs) and saved for offline processing and analysis. Total acquisition time for 10 repetitions (150images/camera) was approximately 3 min. Reflectance at each spatial frequency and wavelength was calculated using

Eq. (2)

Rfx=(21/2/3)×[(I1I2)2+(I1I3)2+(I2I3)2]1/2,
where Rfx is the demodulated frequency amplitude image and I1, I2, and I3 are the three captured images at three different phases, respectively, collected by each camera. Rfx images were further calibrated using silicone phantoms having known optical properties to correct for factors inherent in the instrument, such as inhomogeneous light distribution in the imaging plane or lens aberration. A Levenberg–Marquardt algorithm was used to solve for values of μa and μs that best matched the frequency-dependent sample reflectance to calculated values from a scaled Monte Carlo model of light transport.23

Fig. 1

Diagram of flexible LED and modulation element experimental imaging setup (from Ref. 24). All components were controlled with LabVIEW software on a personal computer.

NPH_2_4_045001_f001.png

2.3.

Correction Factor for Baseline μa

SFDI relies on a model of light transport that assumes absorbers are homogeneously distributed. However, our sample measurements are of highly absorbing hemoglobin packed into blood vessels in the brain. Given a sufficiently large vessel radius, μa can be underestimated due to light not interrogating the whole cross section of a vessel. An established correction factor C(λ)25,26 was used to account for this

Eq. (3)

C(λ)=1exp[2*μa,b(λ)*Rvessel]2*μa,b(λ)*Rvessel,
where μa,b(λ) is the average absorption coefficient of whole blood inside blood vessels and Rvessel is the average vessel radius in the interrogated volume. We calculated μa,b(λ) with an estimated hemoglobin oxygen saturation of 50% and a normal concentration of 2062μM,27 using 64,500g/mol as the molecular weight of a hemoglobin tetramer molecule. Mouse cortical capillaries are 4 to 6μm28 in diameter and surface vessels are 100 to 400μm29 in diameter. Average cortical Rvessel was estimated to be 15μm,30,31 leading to calculated correction factor values of 0.79 at 460 nm and 0.76 at 530 nm. The uncorrected μa was divided by the appropriate correction factor, and this is the value reported in the results and used in all further analyses.

At 460 and 530 nm, the absorption coefficients of cytochrome, lipids, and water are several orders of magnitude smaller than the absorption coefficients for oxyhemoglobin and deoxyhemoglobin.3235 With only two wavelengths measured, we chose to fit for the two dominant absorbers, oxy- and deoxyhemoglobin. Baseline oxy- and deoxyhemoglobin values were calculated from the corrected μa values using least-squares linear fit of the Beer–Lambert law as before.24

2.4.

Multispectral Imaging of Evoked Stimulus Response

Following SFDI data acquisition, the FLaME system was used to measure the NVC response to hindpaw stimulation. Light at 460 and 530 nm was continuously projected onto the mouse brain with an AAXA microprojector, and images were acquired at a frame rate of 2 Hz. An experiment consisted of ninety 30-s trials, with each trial consisting of 2 s of baseline followed by 2 s of 10 Hz, 1-ms electrical pulse stimulation, and 26 s of monitored response time. The electrical stimulus was sent through two needle electrodes inserted subcutaneously into the palmar surface of the left hindpaw of a mouse between the first and second digits and the fourth and fifth digits. An isolated stimulator (World Precision Instruments), controlled by TTL pulses sent from a data acquisition board (National Instruments), adjusted the stimulation current needed to elicit a hindpaw twitch (average 1.5 mA). We averaged the ninety 30-s trials in order to determine the average change in reflectance for each mouse.

2.5.

Hemoglobin Change Calculations

Hemoglobin concentration changes were calculated using the MBLL with both a literature-derived DPF for mouse brain and SFDI-derived DPF. The DPF is a function of μa and μs of the sample, and it was solved for using the method previously described.36 We employed Monte Carlo simulation code23 assuming uniform illumination, a tissue refractive index of n=1.43, and a scattering anisotropy factor of g=0.9. The DPF estimates from Monte Carlo simulations only require inputs of μa and μs, which are measured by SFDI with no prior knowledge of possible chromophores and scatterers. To calculate the literature-derived DPF, we used common assumptions of absorption (μa,460nm=0.83mm1, μa,530nm=0.91mm1, reflecting 60μM HbO2 and 40μM Hb in the brain) and wavelength-independent scattering (μs460and530nm=1.0mm1) in mice.37,38 DPF has units of optical density (OD) mm.

2.6.

Data Analysis

Total hemoglobin (THb) was calculated as the sum of HbO2 and Hb. Oxygen saturation (O2 sat) was calculated as (HbO2/THb)×100. ROIs were selected manually to include the hindpaw somatosensory cortex contralateral to the side of stimulation [Fig. 2(a)], and ROI pixel values were averaged for each mouse. Further analyses used average ROI values for each mouse, and error bars are standard error between mice in each group. p values were calculated using a Student two-tailed t-test. All analyses were done in MATLAB.39

Fig. 2

Spatial frequency domain imaging (SFDI)-measured μa and μs are different from literature-reported values at 460 and 530 nm. (a) A region of interest (ROI, red square) was selected over the contralateral somatosensory cortex of the stimulated hindlimb. (b) The average and standard error of ROI values in each group are shown for μa and compared to a literature-reported value.37 (c) The average and standard error of ROI values in each group are shown for μs and compared to a typically assumed literature-reported value of 1mm1.38

NPH_2_4_045001_f002.png

3.

Results

3.1.

Baseline SFDI Measurements Compared to Literature-Reported Values

Absorption contrast was seen between the 3xTg-AD mice and controls at 460 and 530 nm [Table 1 and Fig. 2(b)]. Compared to assumed values in the literature, measured mean μa values for control and 3xTg-AD mice at 460 nm were 1.2% and 22% lower, respectively; at 530 nm, they were 22% and 40% lower than assumed values. Moreover, mean absorption values differed significantly between control and 3xTg-AD mice at 460 and 530 nm. A wavelength-dependent linear least-squares fit of μa values to the Beer–Lambert law allowed us to calculate HbO2, Hb, THb, and O2 sat for control versus 3xTg-AD mice [Table 2 and Fig. 3(b)]. These results of lower THb and HbO2 in the 3xTg-AD mice are in agreement with our previous measurements using NIR wavelengths.14,15 This likely reflects decreased blood vessel density and volume in 3xTg-AD mice compared to age-matched control mice, as we have shown previously.15

Table 1

Comparison of baseline optical properties and differential pathlength factor (DPF) for control and 3xTg-AD mice.

Control3xTg-ADp value
μa (460 nm)0.82±0.05mm10.65±0.05mm10.04
μa (530 nm)0.71±0.04mm10.55±0.04mm10.02
μs (460 nm)1.5±0.1mm11.7±0.1mm10.21
μs (530 nm)1.3±0.1mm11.5±0.1mm10.21
DPF (460 nm)0.5813±0.0408 OD mm0.6358±0.0365 OD mm0.38
DPF (530 nm)0.6562±0.0327 OD mm0.7293±0.0455 OD mm0.21

Table 2

Comparison of baseline hemoglobin fits for control and 3xTg-AD mice.

Control3xTg-ADp value
HbO277±7μM60±4μM0.10
Hb8±4μM2±1μM0.27
THb85±4μM62±5μM0.004
O2 sat90±5%96±2%0.38

Fig. 3

(a) The average and standard error of ROI values in each group are shown for differential pathlength factor (DPF) and compared to a literature-reported value.36 (b) The average and standard error of HbO2, Hb, THb, and O2 sat in each group are shown and compared to typically literature-reported values.37,38

NPH_2_4_045001_f003.png

Compared to controls, μs averaged 14% higher in the 3xTg-AD mice, but the difference was not significant [Table 1 and Fig. 2(c)]. SFDI-derived DPF was calculated from the μa and μs maps using Monte Carlo simulations. For control and 3xTg-AD mice, mean DPF values at 460 nm were 8.7% and 0.14% lower, respectively, than the literature-derived DPF (0.6367 OD mm); at 530 nm, they were 3.7% and 9.7% higher, respectively, than the literature-calculated value (0.6328 OD mm) (Table 1). In the 3xTg-AD mice, the decrease in μa and increase in μs compared to controls have opposing effects on DPF such that the difference in DPF values was statistically insignificant between 3xTg-AD mice and controls.

3.2.

Qualitative Difference in Blood Overperfusion Between 3xTg-AD and Control Mice

Before a quantitative MBLL calculation of hemoglobin change was performed, the normalized reflectance change in the two wavelengths was averaged across all 30-s trials. Increased blood flow from brain activation could be seen in the ROIs with 530-nm reflectance, since that is an isosbestic point for HbO2 and Hb absorption (Fig. 4). The overperfusion response lasted longer in the controls compared to 3xTg-AD mice. Interestingly, an overperfusion response to activation was seen bilaterally and diffusely, differing from the more localized response seen in rats.40 Others have seen localized responses in mice with whisker stimulation.41 However, another group has also observed a bilateral diffuse response to hindpaw stimulation,42 and it may be due to bilateral activation of pain pathways to the thalami with a larger stimulus.43

Fig. 4

Representative montage of the normalized reflectance change at 530 nm in the ROI (grayscale image) for (a) controls and (b) 3xTg-AD mice. Stimulation started after 2 s of baseline, lasted for 2 s, and images every 2 s subsequently are shown up to 30 s. Color scale shows normalized changes from 4% to 4%. Scale bars (grayscale images) are 1 mm.

NPH_2_4_045001_f004.png

3.3.

Effect of Calculating Hemoglobin Concentration Changes Using the MBLL with SFDI-Derived Versus Literature-Derived DPFs

The hemodynamic changes resulting from the MBLL equation can be seen as group average tracings in Fig. 5, either with the SFDI-derived DPF [Figs. 5(a) and 5(b)] or the literature-reported DPF [Figs. 5(c) and 5(d)]. The time course of the vascular response in control mice agreed with previous studies in mice.42,44 Interestingly, previous multispectral imaging of forepaw stimulation in mice showed an increase in Hb and THb with a decrease in HbO2,44 but our hindpaw stimulation results show an increase in HbO2 and THb with a decrease in Hb, agreeing with studies in rats and most functional activation studies.40,44,45

Fig. 5

Average tracings of HbO2 (red lines) and Hb (blue lines) changes in controls (solid lines) and 3xTg-AD mice (dotted lines). Stimulation occurred from 2 to 4 s (shaded area) and standard error bars are shown. (a) Absolute hemoglobin changes calculated from the MBLL using SFDI-measured baseline optical properties. (b) Percentage hemoglobin changes calculated from the MBLL using SFDI-measured baseline optical properties. (c) Absolute hemoglobin changes calculated from the MBLL using literature-reported baseline optical properties. (d) Percentage hemoglobin changes calculated from the MBLL using literature-reported baseline optical properties.

NPH_2_4_045001_f005.png

In control mice, hemodynamic changes calculated from the MBLL using the SFDI-derived DPFs show a 38% increase in peak HbO2 and threefold decrease in Hb changes compared to calculations using assumed DPFs [Figs. 6(a) and 6(d)]. Most notably, the overall change in Hb during the overperfusion (i.e., area-under-the-curve from 4 to 30 s) was calculated as a slight increase with assumed DPFs, as opposed to the expected decrease with SFDI-derived DPFs (4±7μMs versus 23±5μMs, p<0.0001) [Figs. 6(b) and 6(e)]. These differences suggest deriving DPFs from actual optical property baselines has a large impact on the calculated hemoglobin change.

Fig. 6

Three parameters are compared that describe the overperfusion response in the mice. Graphs in (a), (b), and (c) are calculated in the case where the MBLL was informed with assumed DPFs, based on optical property values commonly used by other groups. Graphs in (d), (e), and (f) are calculated in the case where the MBLL was informed with SFDI-derived DPFs. (a, d) Peak hemodynamic change was determined by finding the largest concentration change from baseline of HbO2, Hb, and THb after the 2-s stimulation. (b, e) Area-under-the-curve (AUC) of the overperfusion after the 2-s stimulation was calculated by integrating the average hemoglobin concentration changes from 4 to 30 s. (c, f) Relaxation time T50 of the hemodynamic response was calculated as the time the overperfusion returns to half its peak value. *p<0.05 for both assumed DPF baseline and SFDI-derived DPF baseline for hemodynamic fitting. **p<0.05 for SFDI-derived DPF baseline and not assumed DPF baseline.

NPH_2_4_045001_f006.png

By comparing control to 3xTg-AD mice with SFDI-derived DPFs for the MBLL calculations, we saw a threefold larger and twofold longer period of overperfusion with HbO2 [Figs. 6(e) and 6(f)]. Furthermore, SFDI correction of the DPF revealed a sevenfold larger reduction in Hb washout in controls compared to AD mice [Fig. 6(e)]. This reduction would have been masked had assumed DPF values been used [Fig. 6(b)]. This is the first study, to our knowledge, showing hemodynamic response in a mouse model of AD to evoked stimuli, and these results support the hypothesis that a diminished hemodynamic response to a metabolic demand is part of the pathogenesis of AD.

4.

Discussion

This is the first measurement of baseline optical properties in an AD mouse model and age-matched controls to inform the MBLL during an evoked stimulus experiment. We found that the baseline optical properties probed by visible wavelengths (460 nm, 530 nm) substantially differ from previously published values of μa and μs.37,38,40 Most notably, the mean baseline cortical oxygen saturation was 90% and 96% for control and 3xTg-AD mice, respectively. Previously published work in the same mouse model, when using an NIR-SFDI instrument, found baseline HbO2 and Hb for 20-month-old control mice were 77 and 58μM, respectively (oxygen saturation 57%); baseline HbO2 and Hb for 20-month-old 3xTg-AD mice were 58 and 49μM, respectively (oxygen saturation 54%).15

This implies that either the visible-SFDI-measured saturations are inaccurate or we are accurately measuring superficial cortical oxygen saturations, which differ from deep cortical saturations interrogated with NIR. Anatomically, highly oxygenated superficial arterioles dive deep into the cortex. Other investigators have found partial pressure of oxygen to be 50 mm Hg up to a depth of 250μm into the cortex, and it decreases with depth in the mouse brain.46,47 It has also been shown under normal imaging conditions that the cortex can be up to 10°C lower than body temperature,48 potentially left-shifting the hemoglobin oxygen-dissociation curve of a mouse49 to account for 90% O2 sat. Future experiments should monitor and control blood gas, heart rate/cardiac output, and brain surface temperature to better validate these results. Multilayer heterogeneous light tissue transport modeling combined with fMRI could also inform and validate these preliminary results.50

One possible source of error is the μa correction factor, C(λ). C(λ) of NIR measurements is effectively 1 since the μa of whole blood in the NIR is 100-fold lower than in the visible. However, at the hemoglobin isosbestic point 530 nm, C (530 nm) can range from 0.96 to 0.13 for an average vessel radius of 2 to 200μm, respectively. Therefore, the total hemoglobin measurements depend on the average vessel radius, which are not measured or known in our mouse model. C (460 nm) could also potentially affect the baseline oxygen saturation estimation. In post-hoc analysis, sweeping the assumed average vessel radius (15 to 150μm) and average oxygen saturation (30 to 100%) of whole blood did not correct the SFDI-measured raw μa values to match literature-reported oxygen saturations with appropriate total hemoglobin concentrations. Large differences in cytochrome and lipid concentration could also alter the baseline hemoglobin concentration and saturation calculations. Cytochrome oxidase activity is reduced as much as 26% and 50% in human postmortem AD brain tissue51 and platelets,52 respectively. Future experiments that use multiple (>3) wavelengths to fit for additional chromophores could potentially address this issue.

Wavelength-dependent scattering measured with visible-SFDI was also attenuated when compared to NIR-SFDI measurements.15 Reduced scattering coefficients ranged from approximately 1.6 to 1mm1 from 650 to 970 nm, respectively. In the NIR, wavelength-dependent scattering was significantly higher in 3xTg-AD brains compared to controls, which could be attributed to morphological changes caused by an increased inflammatory state.19 We hypothesize the plateauing of μs at 460 and 530 nm may be partially explained by the limited penetration depth of visible wavelengths versus NIR wavelengths53 and the layered nature of the mouse skull and brain. Ex vivo from 460 to 530 nm, the brain is highly scattering (μs6mm1)54 while the skull (μs3.5mm1) and CSF are less so.55,56 In the visible wavelength regime, the thinned skull and underlying cerebral spinal fluid take up a higher partial volume fraction, diluting the perceived scattering from the brain. Differences in thin skull preparation, as well as the effect of saline on the skull,24 could also account for the variability in μs measured with visible-SFDI, ameliorating the difference between control and 3xTg-AD brains seen with NIR-SFDI.

The percentage change line graphs in Figs. 5(b) and 5(d) highlight a major difference in how we would interpret the underlying biology of the optical signals. Such high baseline oxygen saturation would significantly affect a calculation often used to approximate relative change in brain metabolism during an evoked stimulus response, the relative change in cerebral metabolic rate of oxygen (rCMRO2) [Eqs. (4) and (5)]:37,38

Eq. (4)

γ=ΔHbTνHbTνo/ΔHbTHbTo,

Eq. (5)

1+rCMRO2=(1+ΔCBFCBFo)(1+ΔHbHbo)(1+γΔHbTHbTo)1,
where rCMRO2 is equal to the fractional change in cerebral blood flow (CBF) (typically measured with laser speckle imaging) multiplied by the fractional change in Hb and divided by the fractional change in THb in the veins. Specifically, in our measured evoked stimulus response, the fractional decrease in Hb (e.g., 50%) would overwhelm the smaller fractional increase in CBF (e.g., typically +10%) and produce a negative change in local metabolism. It is unclear how to integrate these baseline oxygen saturation measurements into the rCMRO2 calculation, but this hypothetical scenario underscores the importance of accurate knowledge of baseline conditions to interpret multispectral imaging results.

In our evoked stimulus experiment, relatively large signals such as the HbO2 overperfusion were qualitatively the same regardless of using assumed or SFDI-informed DPFs. We replicated the attenuated overperfusion response that has been well characterized with laser speckle imaging in a CAA mouse model,16 NIR spectroscopy measurements in AD patients,11,12 and fMRI blood-oxygen-level dependent (BOLD) response in a rat model of AD.57 The fMRI BOLD response has also been shown to be sensitive to loss of clinically significant tasks in patients with AD, such as face recognition58 and stepwise loss of smell with worsening AD.59 However, task-based fMRI BOLD signal interpretation is limited by fluctuations in the baseline perfusion and metabolic states of the brain.60,61 Our work in characterizing the 3xTg-AD baseline optical properties indicates that there are global and spatial differences in oxygen saturation compared to control brains. Knowledge of the baseline optical properties allowed detection of Hb washout differences during an evoked stimulus in 3xTg-AD mice. In the context of early detection of AD, our work supports the use of resting state fMRI, arterial spin labeling, and PET/SPECT to fully characterize baseline perfusion and metabolism differences in AD that may then further inform task-based fMRI BOLD signals.

In conclusion, this work emphasizes the differences in baseline optical properties from typically assumed values when studying metabolism in rodents with varying thin skull thicknesses, comparing disease models of AD, and using visible versus NIR wavelengths in multispectral imaging. We have shown that SFDI-derived DPF allows for more accurate determination of hemodynamic response to an evoked stimulus in an AD mouse model.

Acknowledgments

Funding for this work was supported in part by the NIH (NIBIB) Laser Microbeam and Medical Program (P41EB015890), NIH (NIBIB) Grant (R21 EB014440), NIH (NINDS) Grant (R21 NS078634), NIH (NIA) Grant (R01 A6-21982), NIH (NIA) Ruth Kirschstein NRSA fellowship (5F30AG039949-02), U.C. Irvine MSTP, and the Arnold and Mabel Beckman Foundation.

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Biography

Alexander J. Lin is a graduate of the Medical Scientist Training Program at the University of California, Irvine (UCI). He received his PhD from the Department of Biomedical Engineering at UCI in 2013, working in the lab of Bruce Tromberg at the Beckman Laser Institute. He plans to continue his residency training in radiation oncology at Washington University in St. Louis.

Adrien Ponticorvo is a project scientist at the Beckman Laser Institute at UCI. His research is focused on the development and application of novel optical imaging techniques to quantify physiology. He holds a PhD in biomedical engineering from the University of Texas at Austin with an emphasis on biomedical optics.

Anthony J. Durkin is an associate professor at the Beckman Laser Institute at UCI. His research is focused on the development and application of optical spectroscopic and quantitative wide-field imaging techniques to characterize superficial tissues in vivo. He is codirector of the wide-field functional imaging program at BLI. He holds a PhD in biomedical engineering from the University of Texas at Austin with emphasis on biomedical optics and spectroscopy.

Vasan Venugopalan is a professor and chair of the Department of Chemical Engineering and Materials Science at UCI, with a joint appointment at Beckman Laser Institute. He received a BS degree from the University of California, Berkeley and a ScD degree from Massachusetts Institute of Technology, both in mechanical engineering. His current research is in the areas of computational biophotonics and the interaction of pulsed laser microbeams with cells and tissues.

Bernard Choi is an associate professor of biomedical engineering and surgery at UCI, with appointments at Beckman Laser Institute and Medical Clinic, Edwards Lifesciences Center for Advanced Cardiovascular Technology, and the Children's Hospital of Orange County. His current research is in the field of vascular biophotonics, with current emphases in optical hemodynamic monitoring, microvascular dynamics of tissue in normal and proliferative states, and optical clearing.

Bruce J. Tromberg is the director of the Beckman Laser Institute and Medical Clinic at UCI and principal investigator of the Laser Microbeam and Medical Program (LAMMP), an NIH P41 National Biomedical Technology Center in the BLI. He is a professor with joint appointments in the Departments of Biomedical Engineering and Surgery and has been a member of UCI faculty since 1990.

© 2015 Society of Photo-Optical Instrumentation Engineers (SPIE) 2329-423X/2015/$25.00 © 2015 SPIE
Alexander J. Lin, Adrien Ponticorvo, Anthony J. Durkin, Vasan Venugopalan, Bernard Choi, and Bruce J. Tromberg "Differential pathlength factor informs evoked stimulus response in a mouse model of Alzheimer’s disease," Neurophotonics 2(4), 045001 (12 October 2015). https://doi.org/10.1117/1.NPh.2.4.045001
Published: 12 October 2015
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