18 August 2017 In vivo measure of neonate brain optical properties and hemodynamic parameters by time-domain near-infrared spectroscopy
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By exploiting a multichannel portable instrument for time-domain near-infrared spectroscopy (TD-NIRS), we characterized healthy neonates’ brains in term of optical properties and hemodynamic parameters. In particular, we assessed the absolute values of the absorption and reduced scattering coefficients at two wavelengths, together with oxy-, deoxy- and total hemoglobin concentrations, and the blood oxygen saturation of the neonates’ brains. In this study, 33 healthy full-term neonates were tested, obtaining the following median values: 0.28 and 0.31  cm−1 for μa at 690 and 820 nm, respectively; 5.8 and 5.3  cm−1 for μs′ at 690 and 820 nm, respectively; 103  μM for cO2Hb; 42.6  μM for cHHb; 146  μM for ctHb; 72% for sO2. In general, the agreement of these values with the sparse existing literature appears not always consistent. These findings demonstrate the first measurements of optical properties of the healthy neonate brain using TD-NIRS and show the need for clarification of optical properties across methods and populations.



Near-infrared spectroscopy (NIRS) is an optical technique offering a noninvasive and safe method to study the human brain, allowing both a functional approach and the evaluation of its optical (absorption and reduced scattering coefficients μa and μs, respectively) or physiological baseline characteristics. Since Jöbsis1 demonstrated that, by exploiting the transparency of biological media to the near-infrared light, it was possible to assess the oxygenation of cerebral tissues, an increasing interest has been focused on the use of NIRS for adult and infant brain measurements.2 In fact, if one considers the hemoglobin as the main absorbing chromophore at the operating wavelengths, the information about oxy- and deoxyhemoglobin concentrations (cO2Hb and cHHb, respectively) inside a tissue can be easily derived from the measurement of its absorption properties. Furthermore, because the light diffuses inside tissues, measurements in reflectance configuration are possible, making feasible a flexible and experiment-designed configuration of optodes.

In most experiments presented in literature, the NIRS setup is based on a continuous wave (CW) approach. Despite some advantages of this method, the CW technique is intrinsically not able to retrieve separately the absorption and scattering properties of the investigated tissue: from CW data, it is possible to determinate only a tissue oxygenation index, which is the product μa×μs, considered as a “scaled absolute absorption coefficient.”3 This limitation of CW approach can be overcome considering a multidistance approach without trivial preliminary calibration of the instrument.4 In general, a CW NIRS device cannot give information about the absolute values of the optical parameters, but it can detect only variations of the absorption properties of tissues. Then, the CW approach is appropriate to study the functional behavior of the brain, where experimental protocols are usually designed to detect hemodynamic changes. On the contrary, it is not suited for absolute quantification of the baseline optical characteristics without any assumption on μs.

A second approach to NIRS is based on the use of light pulses hundreds of picoseconds broad.5 The time-domain (TD) approach to NIRS allows the reconstruction and the distribution of time-of-flight (DToF) of the detected photons. By exploiting proper physical models for photon migration,6 from the measured time-resolved reflectance (TRR) curves, it is possible to discriminate between tissue optical properties assessing the absolute values of μa and μs.

A third approach is the frequency-domain (FD) NIRS, where the source is a radiofrequency modulated light, and the measures of the phase shift and light attenuation are considered. If a sufficient number of modulational frequencies are simultaneously used, in principle, the absolute optical properties of the investigated tissue can be retrieved, this approach being equivalent through a Fourier transformation to the TD-NIRS. However, this is not usually the case and the use of more than one modulational frequency has to be considered such as an exception.7

Application of NIRS is particularly suitable and attractive on infants first because of the safe, noninvasive, and wearable characteristics of this technique, as well as for its low cost and portability. In fact, NIRS can integrate or substitute other imaging modalities that are usually limited in studying neonates, children, or, in general, subjects without a full control of movements. Furthermore, the thinner skull thickness of infants’ heads with respect to adults improves the sensitivity on brain regions, making NIRS experiments on babies particularly successful. In particular, because the overall thickness of scalp and skull in neonates can be estimated of about 5 mm,8 assumptions on the head geometry can be simplified by modeling it as a homogeneous semi-infinite medium. This approximation is not valid for adults, where multilayered models have to be considered to account for the real structure of the head.9

Several functional experiments have been carried out on babies both for research or clinical activities, and they are mainly focused on their cognitive or behavioral development, using NIRS to measure tissue oxygenation associated with neural activity.1011.12.13 Conversely, up to now, only few studies have been performed by NIRS about in vivo baseline optical or physiological properties of the neonate brain. This is mainly due to the fact that most of the commercially available systems for NIRS are based on CW technology, and thus present the limitations described above. For instance, the group of Zhao et al.14 used a FD-NIRS setup to measure baseline optical properties and, from them, they recovered the concentrations cO2Hb and cHHb of the two hemoglobin species, oxy- and deoxyhemoglobin, respectively, the concentration ctHb of the total hemoglobin content, and the oxygen saturation sO2. A monitoring experiment on neonates about the brain development (in terms of ctHb, sO2, blood volume, and cerebral metabolic rate of oxygen) was performed by Franceschini et al.15 and shows consistency with previous positron emission tomography and electroencephalogram studies. In a more recent paper by a Swiss group (see Ref. 16), they were studied, by exploiting a FD-NIRS system, the effects of the homogeneity assumption of infant head on the precision of hemoglobin concentration measurements. Baseline absorption and scattering parameters are also tabulated therein. A study of brain optical properties at different ages (from neonates to adults) and with different geometry models was conducted by a Harvard research group by using a FD system for NIRS.9

To our knowledge, only one study about the baseline characterization of neonate brain performed by TD-NIRS has been published in literature until now,17 where researchers provided data of μa, μs, sO2, and cerebral blood volume. They also studied a possible relationship between these variables and the neonate postconceptional age. However, the population of neonates they considered was undergoing neonatal intensive care. In this paper, we report about a study, where TD-NIRS is employed on healthy full-term neonates, in order to determine the brain optical properties μa and μs, and hemodynamic parameters cO2Hb, cHHb, ctHb, and sO2. The ultimate goal of this study is to provide the scientific community with a further and numerically consistent dataset concerning the characterization of the neonate healthy brain from the optical, i.e., in the visible and near-infrared spectral range, point of view.


Materials and Methods



Thirty-three healthy full-term neonates (13 male and 20 female, 3.2±0.9 days after birth) underwent the measurements at the Presidio Ospedaliero Universitario “Santa Maria della Misericordia” in Udine, Italy. All infants had an Apgar score higher than 7 at 1 and 5 min after birth and no cephalohematomas. Clinical details of neonates are summarized in Table 1. Written informed consent was signed by parents of every neonate prior to the enrollment, and the study was approved by the local ethics committee.

Table 1

Clinical details of the enrolled neonates. M: male; F: female; NA: not available; SD: standard deviation.

Neonate (#)GenderAge (days)Gestational age (weeks)Weight (kg)Circumference head (cm)


TD-NIRS Instrument

Measurements have been performed by the class I medical device for TD-NIRS developed at the Physics Department of Politecnico di Milano. The light sources are two pulsed diode lasers (Picoquant GmbH, Germany) operating at 690±10  nm and 820±10  nm, with a repetition rate of 80 MHz, pulse duration of about 100 ps, and average power less than 2 mW. The laser pulses are coupled into two multimode graded index fibers (50/125  μm core/cladding diameter) of different length and time multiplexed by means of a 2×2 fused fiber optics splitter (OZOptics, Canada), in order to create two independent sources. Then, a couple of 1×9 fiber optics switches (PiezoJena GmbH, Germany) are used to create up to 16 independent injection points (one channel in each optics switch is sacrificed to create a stopping channel). The light power delivered on the sample is far less than 1 mW, 15 times below the safety limit.

The detection of remitted photons, after propagation in the diffusive medium, is accomplished by four parallel and identical detection chains. Each chain presents the combination of a compact four-channel photomultiplier (Hamamatsu Photonics, Japan), a home-made amplifier with variable gain, and routing electronics (Becker&Hickl, Germany). All the photomultipliers are cooled by means of Peltier units and metal heat sink to reduce thermal noise. All the 16 detection channels are equipped with an independent variable attenuator in order to equalize the signal coming from different areas of the sample. The registration of the DToFs is performed by four time-correlated single-photon counting (TCSPC) boards (Becker&Hickl, Germany). More details about the instruments and their performances are described in Ref. 18.

We note that the system can manage up to 16 injection points and up to 16 collection points. Furthermore, it can be used with different types of collecting fibers or fiber bundles. For the present experiment, we adapted the device in order to use as probes two soft black silicon cushions, into which small fiber bundles (1 mm in diameter), made of step-index optical fibers, for light delivery and detection, are embedded (see Fig. 1). These probes were light and comfortable, and the soft silicon cushions allowed an optimized contact with the infant scalp. Then a subset of five injection fibers and four detection channels were used in each silicon pad, providing 12 source–detectors pairs on each probe (overall 24 registered channels). The distance between detectors and receivers was 3 cm. In order to limit the temporal spread of light pulses due to their propagation into the fiber bundles, the latter ones were kept as short as possible, setting a final length of about 2.5 m. In this way, the instrument response function (IRF) of the device is on the order of 500 ps for FWHM (compare Fig. 2).

Fig. 1

Silicon probe use for the measurements on the neonates’ heads. Red squares: injection points; blue squares: detection points. (a) External side and (b) surface in contact with the head (fiber tips are visible).


Fig. 2

(a) It is reported a typical TRR curve (red squares) recorded at 690 nm with the TD-NIRS device, together with the corresponding IRF (green squares) and the model resulting from fitting procedure (yellow line). The FWHM of IRF is 640 ps, while the number of photons of the TRR curve is about 5×105. The vertical light blue lines mark the range considered for the best fit of the model to the data: the retrieved optical properties in this case are μa=0.26  cm1 and μs=7.7  cm1. (b) The residuals of the fitting procedure are reported, calculated as (OiMi)/Mi, where Oi is the i’th observation and Mi is the correspondent calculated model (see main text). From residuals, the reduced χ2 value of the fitting procedure can be calculated resulting in this case 3.22.


The source fibers were sequentially illuminated every 0.2 s in the left and right hemispheres in parallel, for an overall acquisition time of 1 s (1-Hz sampling rate for a full topographic map).


Measurement Protocol

Infants were tested in a quiet and dimmed room within their cribs. One silicon probe was placed over the right hemisphere, the other over the left one, in such a way to cover from the frontal area until the central areas of the neonates’ heads, centered on the perisylvian areas. The pads were fixed by a sterile bandage; moreover, an experimenter kept his hands on the probes for all the duration of the experiment in order to assure the best contact between scalp and optodes by following the infant’s head movements. The TD-NIRS device was positioned outside the room, and the acquisitions were observed via infrared video projection to an external monitor. A medical doctor was present in the room during the experiment to soothe the infant if necessary. After waiting a while for the complete adaptation of the infant to the environment, we proceeded with the acquisition of TD-NIRS signal, lasting 30 s.

The raw data collected in this study are not available to the community as open access data.


Data Analysis

Since in TCSPC technique, the Poisson noise, affecting the measures, is proportional to the square root of the acquired photon number, the TRR curves registered during the 30 s of acquisition were summed up for each measurement point, in order to increase the photon counts and thus improve the signal-to-noise ratio (SNR). Next, the obtained TRR curves were fitted using the solution of the diffusion equation for a semi-infinite homogenous diffusive medium obtained considering extrapolated boundary conditions,6 in order to retrieve the absorption and reduced scattering coefficients for each neonate’s brain at the two considered wavelengths. The refractive index of the brain tissue was fixed to 1.4. As a matter of fact, taking into account the very small thickness of scalp and skull in neonates (about 5 mm8) and the source–detector distance considered in the measurements (3 cm), the retrieved values for the absorption and reduced scattering coefficients are essentially those of the cerebral tissue.19 An exemplum of the results of the fitting procedure is reported in Fig. 2.

Once obtained the absorption coefficients at the two different wavelengths 690 and 820 nm, the absolute values of the oxy- and deoxyhemoglobin concentrations can be calculated by exploiting the Beer–Lambert’s law. If we assume that the oxy- and deoxyhemoglobin, together with water, are the main absorbers of the head tissues in the spectral range considered, then it results:


where μaλ are the absorption coefficients at a given wavelength λ, εXλ are the extinction coefficients of the chromophores X (oxy- and deoxyhemoglobin and water) at a given wavelength λ, and cX are the concentrations of the chromophores X. We explicitly note that here we are considering the adult hemoglobin. Even if in neonates’ blood also the fetal hemoglobin is present, it rapidly declines in the first weeks of life in favors of the adult hemoglobin. Furthermore, the absorptivities of fetal and adult hemoglobin result indistinguishable in the spectral range of interest here, as demonstrated in Refs. 20 and 21. Then, in general, we will refer to the concentrations of the adult hemoglobin derivatives (that is oxy- and deoxyhemoglobin).

Next, we fixed the water concentration cH2O to 90% and inverted the linear system [Eq. (1)] in order to get the oxy- and deoxyhemoglobin content, cO2Hb and cHHb, respectively.22 The values we used for the extinction coefficients of the chromophores are reported in Table 2.23

Table 2

Values for the extinction coefficients of the chromophores used for the inversion of Beer–Lambert’s law reported in Eq. (1).

Wavelength (nm)εO2Hb (cm−1 μM−1)εHHb (cm−1 μM−1)εH2O (cm−1)

Finally, the concentration ctHb of the total hemoglobin and the oxygen saturation sO2 can be calculated as follows:






Results and Discussion

Preliminarily, in order to have results as reliable as possible, we introduced two criteria for the inclusion of measurements in the following analysis.

First, we excluded the TRR curves for which the best fitting procedure with the theoretical model (see Sec. 2.4) ends with a figure of merit, i.e., the reduced χ2 value, larger than 10. In particular, in the least square curve fitting procedure adopted here, the reduced χ2 was calculated in the typical way:


where f are the degrees of freedom, i.e., the number N of observations minus the number of retrieved parameters, Oi is the i’th observation, i.e., the number of photons measured in a given time channel (compare Fig. 2), Mi is the correspondent calculated model, and σi is the expected error of the observation Oi. Because in the TCSPC technique, measured data are affected by Poisson noise, we set σi=Mi, where we consider the calculated value instead of the observed value because this guarantees a more stable fitting procedure at low counts.24 According to this definition, the expected value for the reduced χ2 is 1. However, we decide to tolerate reduced χ2 values up to 10: as an exemplum, for the fitting procedure reported in Fig. 2, the value of the reduced χ2 is 3.22.

Second, we assumed that TRR curves have an acceptable SNR only if they have at least 104 counts. Indeed, the accuracy of the fitting procedure is within 5% also at this count level.24

In Fig. 3, it is reported for each measured TRR curve, the reduced χ2 value obtained in the best fitting procedure as a function of the counts.

Fig. 3

Scatter plot in logarithmic scale of reduced χ2 value obtained in the best fitting procedure as a function of the detected photon counts for each measured TRR curve. Red lines represent the thresholds set for data rejection.


We observe that the majority of the measurements (88% for both wavelengths) fulfill the inclusion criteria. In particular, for each of the subjects from 1 to 29, on average, 22.6±1.7 registered channels fulfill the inclusion criteria and, then, will be considered for the following analysis. On the contrary, the neonates 30, 31, 32, and 33 have been totally excluded from data analyses because only a few numbers of registered channels fulfill the inclusion criteria (only 7.5±4.2 for each of these subjects). This is mainly due to the presence of important movement artifacts during the measurements on these neonates.

As for the optical properties, in Fig. 4, they are reported as the median values of absorption and reduced scattering coefficients μa and μs of the neonates’ brains, calculated at two wavelengths by considering all the registered channels for each neonate. Furthermore, in Fig. 5, they are reported as the median values of the oxy-, deoxy- and total hemoglobin concentrations cO2Hb, cHHb and ctHb, and the oxygen saturation sO2 of the neonates’ brains, calculated as in Fig. 4 for each neonate. In order to have an idea of the distribution of values registered on each neonate, in Figs. 4 and 5 the 25th and 75th percentile of such distribution are also reported as error bars. Even if the intrasubject variability, i.e., the variability among the 24 registered channels for each neonate, is important, then the intersubject variability appears prominent. In order to explain the observed intrasubject variability in the measured data, we can note that, besides the physiological variations among different positions of the neonates’ heads, an important role is probably played by the head curvature, which also varies and could affect the recovered optical properties in different ways. Furthermore, the fact that the probe has been held in position by the operator’s hands could have had some influence, too.

Fig. 4

(a) Median values of absorption coefficient μa and (b) reduced scattering coefficient μs, calculated for different neonates. Error bars represent the 25th and 75th percentile of the values distribution.


Fig. 5

Median values of the (a) oxy-, deoxy- and total hemoglobin concentrations cO2Hb, cHHb, and ctHb and (b) the oxygen saturation sO2, calculated for different neonates. Error bars represent the 25th and 75th percentile of the values distribution.


In order to make the dataset on neonates’ brains more usable, the values of optical properties μa and μs at two wavelengths, and of the hemodynamic parameters cO2Hb, cHHb, ctHb, and sO2, shown in Figs. 4 and 5, are reported in the Appendix (see Table 3). Here, we report the same parameters for the overall neonates’ population. In the format, “median [25th–75th percentile],” we obtained: 0.28 [0.220.34]  cm1 and 0.31 [0.250.40]  cm1 for μa at 690 and 820 nm, respectively; 5.8 [4.6–7.0] and 5.3 [4.26.6]  cm1 for μs at 690 and 820 nm, respectively; 103 [80.2139]  μM for cO2Hb; 42.6 [33.453.6]  μM for the cHHb; 146 [119188]  μM for ctHb; 72% [68%–75%] for sO2.

Table 3

Median values of optical and hemodynamic parameters for different subjects.

Neonate (#)μa (cm−1)μs′ (cm−1)cHHb (μM)cO2Hb (μM)ctHb (μM)sO2
690 nm820 nm690 nm820 nm
Median (25th–75th)0.280.315.85.342.61031460.72

Now, we compare our dataset about optical properties and hemodynamic parameters of neonates’ brains with similar datasets present in scientific literature. First, we have to say that there is little about this issue. In particular, as we stated in Sec. 1, to our knowledge, a TD-NIRS instrumentation has been exploited in only one study about the baseline characterization of neonates’ brains.17 Even though therein the authors considered a population of neonates that underwent neonatal intensive care and the wavelength used was not exactly the same, it is anyway interesting to compare results we obtained with theirs. As for the reduced scattering coefficients, the values obtained here are compatible with results in Ref. 17. On the other hand, the values for absorption coefficients found in this work are larger by about 50% with respect to those found in Ref. 17. Consequently, we also observe here an overestimation for the hemoglobin content. Nevertheless, the blood oxygen saturation results are comparable in the two works.

In a few other papers in literature, the baseline characterization of neonates’ brains has been performed by exploiting a FD-NIRS.1415.16,25 In particular, in Refs. 15, 16, and 25, only the hemodynamic parameters are provided. The total hemoglobin content reported in these works is about 5060  μM, i.e., a value for ctHb that is lower with respect to what we reported here, and similar to what is reported in Ref. 17. On the other hand, again we observed comparable values for the blood oxygen saturation. In Ref. 14, they reported also the optical properties of the neonates’ brain tissue. In this case, the absorption and reduced scattering coefficients at two wavelengths, similar to those used here, are lower and larger, respectively, than the values reported in Table 3.

In summary, the values reported in Table 3 about the optical properties and the hemodynamic parameters of neonates’ brain are not always compatible to similar data reported elsewhere. In particular, we can state that the blood oxygen saturation is comparable with literature data, while the absorption coefficient of neonates’ brains reported here is in general overestimated with respect to literature data. We want to stress that literature data are in general few and the population of neonates considered in the different studies sometimes are not comparable: for example, in this paper, we considered only healthy neonates, while in the cited papers are reported results involving also neonates with some pathology. If we add that the majority of data on neonates’ brains have been obtained with a different technique, i.e., the FD-NIRS, we think that there is room for reporting also the dataset presented in this paper and obtained by exploiting a TD-NIRS instrumentation. We hope that further studies on this important issue will be performed also with different techniques, in such a way as to arrive at a reliable, consolidated, and widely accepted dataset about the optical properties and hemodynamic parameters of neonates’ brains.



In this paper, we determined optical properties and hemodynamic parameters of the brain of 33 full-term healthy neonates. An instrument for multichannel TD-NIRS was employed. By adopting fair exclusion criteria for the performed measurements, we obtained a reliable dataset that we compared with a similar dataset already reported in literature that used a different population (newborns in intensive care compared to healthy newborns in our dataset) and a different instrumental approach to NIRS (FD-NIRS as compared to TD-NIRS used here). We found consistencies in the measures of blood oxygenation but variations in the absorption coefficients. However, data concerning the optical characterization of the healthy neonates’ brains in the visible and near-infrared spectral range are few and, for this reason, not conclusive. The data presented here, from a large sample of healthy neonates, demonstrate the optical properties in a healthy newborn’s brain, but show that further research is necessary to clarify how these properties differ by the measurement techniques and population tested.



Neonates’ Dataset Details

In Table 3, we explicitly report the values of optical properties μa and μs at two wavelengths, and of the hemodynamic parameters cO2Hb, cHHb, ctHb, and sO2, already shown in Figs. 4 and 5. In this way, the neonates’ dataset will be more usable and comparisons with other similar datasets, already present in literature or that will be assessed in the future, more straightforward.


No conflicts of interest, financial or otherwise, are declared by the authors.


The research leading to these results has received partial funding from the European Research Council under the European Unions’ Seventh Framework Programme (FP7/2007-2013), ERC Grant Agreement 269502, and from the European Commission, Project BabyLux, Grant Agreement 620996, CIP-ICT-PSP-2013-7.


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Lorenzo Spinelli received his MS and PhD degrees in physics from the University of Milan, Italy, in 1994 and 1999, respectively. Since 1999, he has been a postdoc in the Department of Physics at the University of Milan. In 2001, he became a researcher for Italian Research National Council at Institute of Photonics and Nanotechnologies. His research interests are devoted to the study of photon migration in turbid media for optical biopsy and imaging.

Lucia Zucchelli graduated in biomedical engineering from Politecnico di Milano in 2009 and obtained her PhD in physics at the Physics Department, Politecnico di Milano, in 2013. Her research field is focused on the development and applications of time-resolved optical systems based on near-infrared spectroscopy for functional imaging of the brain. She teaches physics at the high school level.

Davide Contini received his MS degree in electronic engineering and his PhD in physics from Politecnico di Milano in 2004 and 2007, respectively. He is an associate professor in the Department of Physics, Politecnico di Milano, Italy. He is the author of more than 150 papers in international peer-reviewed journals and conference proceedings. His research activity is focused on time-resolved spectroscopy of highly diffusive media for applications in biology and medicine.

Matteo Caffini received his master’s degree in biomedical engineering and his PhD in physics from Politecnico di Milano. He is a researcher with interests in medical imaging, functional near-infrared spectroscopy, and data science. During his PhD, he was awarded a Rocca fellowship and conducted research at the Massachusetts Institute of Technology. Before joining CIMeC at the University of Trento, he worked at Politecnico di Milano and at the preclinical imaging facility of KU Leuven.

Alissa L. Ferry received her MS and PhD degrees in cognitive psychology from Northwestern University in Chicago, United States. From 2011 to 2016, she worked as a postdoctoral research at the International School for Advanced Studies in Trieste, Italy. In 2016, she became a lecturer in the faculty of biology, medicine, and health at the University of Manchester in the United Kingdom. Her research interests are in language acquisition, cognitive development, and cognitive neuroscience.

Francesco Macagno, MD, is a pediatrician and neonatologist, past head of the Nonatologic Department, University–Hospital S.M.M., Udine, Italy, in 1997–2010, and past president of the Italian Society of Neonatology (2000–2003). He was a counselor, as pediatrician, of the Italian Ministry of Health in 1996–2013. He is a holder of the gold medal about merits acquired with regard to the personal contributions in the National Public Health Programs. He is a neonatologist of the SISSA Team Trieste, Italy, regarding the PASCAL Project on the acquired language in neonatal age in 1996–2016.

Luigi Cattarossi, MD, received his medical degree at the School of Medicine of Genoa University. He received his Italian medical license in November 1978. He received a residency in pediatrics on July 15, 1986. He was a fellow in neonatology in November 1987, a director of the neonatal intensive care unit and neonatal nurseries at Udine University Hospital from January 2011 till now, and a professor of neonatal medicine at Udine School of Medicine.

Alessandro Torricelli is a full professor in the Department of Physics, Politecnico di Milano (Italy). He received his MS degree in electronic engineering from Politecnico di Milano in 1994, and his PhD degree in physics from Politecnico di Torino in 1999. He is the author of more than 100 papers on international peer-reviewed journals. His current research interests include photon migration in diffusive media, functional near infrared spectroscopy, and noninvasive diffuse spectroscopy with time domain systems.

Biographies for the other authors are not available.

© The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
Lorenzo Spinelli, Lorenzo Spinelli, Lucia Zucchelli, Lucia Zucchelli, Davide Contini, Davide Contini, Matteo Caffini, Matteo Caffini, Jacques Mehler, Jacques Mehler, Ana Fló, Ana Fló, Alissa L. Ferry, Alissa L. Ferry, Luca Filippin, Luca Filippin, Francesco Macagno, Francesco Macagno, Luigi Cattarossi, Luigi Cattarossi, Alessandro Torricelli, Alessandro Torricelli, } "In vivo measure of neonate brain optical properties and hemodynamic parameters by time-domain near-infrared spectroscopy," Neurophotonics 4(4), 041414 (18 August 2017). https://doi.org/10.1117/1.NPh.4.4.041414 . Submission: Received: 30 March 2017; Accepted: 11 July 2017
Received: 30 March 2017; Accepted: 11 July 2017; Published: 18 August 2017

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