Intracellular fluorescence polarization, picosecond kinetics, and light-induced reactions of photosensitizing porphyrins

Harold K. Seidlitz; Kurt Stettmaier; Jurina M. Wessels; Herbert Schneckenburger

doi:10.1117/12.57692

1 July 1992 Intracellular fluorescence polarization, picosecond kinetics, and light-induced reactions of photosensitizing porphyrins

Harold K. Seidlitz, Kurt Stettmaier, Jurina M. Wessels, Herbert Schneckenburger

Author Affiliations +

Optical Engineering, Vol. 31, Issue 7, (July 1992). https://doi.org/10.1117/12.57692

Abstract

Porphyrin monomers, dimers, and aggregates with various tumor- localizing and photosensitizing properties can be differentiated based on their fluorescence lifetimes. These individual components are shown to possess different degrees of polarization. This fact can be used both for their microscopic imaging and for investigation of the microenvironment of porphyrins within cells and tissues. The spectral bands around 635 and 675 nm were correlated with the emission of porphyrin monomers and aggregates, respectively. The formation of photoprotoporphyrin was not significant during the light-induced porphyrin reaction.

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Harold K. Seidlitz, Kurt Stettmaier, Jurina M. Wessels, and Herbert Schneckenburger "Intracellular fluorescence polarization, picosecond kinetics, and light-induced reactions of photosensitizing porphyrins," Optical Engineering 31(7), (1 July 1992). https://doi.org/10.1117/12.57692

Published: 1 July 1992

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