Phase measurements of erythrocytes affected by metal ions with quantitative interferometric microscopy

Shouyu Wang; Keding Yan; Yanke Shan; Mingfei Xu; Fei Liu; Liang Xue

doi:10.1117/1.OE.54.12.124105

30 December 2015 Phase measurements of erythrocytes affected by metal ions with quantitative interferometric microscopy

Shouyu Wang, Keding Yan, Yanke Shan, Mingfei Xu, Fei Liu, Liang Xue

Author Affiliations +

Optical Engineering, Vol. 54, Issue 12, 124105 (December 2015). https://doi.org/10.1117/1.OE.54.12.124105

Abstract

Erythrocyte morphology is an important factor in disease diagnosis, however, traditional setups as microscopes and cytometers cannot provide enough quantitative information of cellular morphology for in-depth statistics and analysis. In order to capture variations of erythrocytes affected by metal ions, quantitative interferometric microscopy (QIM) is applied to monitor their morphology changes. Combined with phase retrieval and cell recognition, erythrocyte phase images, as well as phase area and volume, can be accurately and automatically obtained. The research proves that QIM is an effective tool in cellular observation and measurement.

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Shouyu Wang, Keding Yan, Yanke Shan, Mingfei Xu, Fei Liu, and Liang Xue "Phase measurements of erythrocytes affected by metal ions with quantitative interferometric microscopy," Optical Engineering 54(12), 124105 (30 December 2015). https://doi.org/10.1117/1.OE.54.12.124105

Published: 30 December 2015

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