Dual-wavelength common-path digital holographic microscopy for quantitative phase imaging of biological cells

Jianglei Di; Yu Song; Teli Xi; Jiwei Zhang; Ying Li; Chaojie Ma; Kaiqiang Wang; Jianlin Zhao

doi:10.1117/1.OE.56.11.111712

11 July 2017 Dual-wavelength common-path digital holographic microscopy for quantitative phase imaging of biological cells

Jianglei Di, Yu Song, Teli Xi, Jiwei Zhang, Ying Li, Chaojie Ma, Kaiqiang Wang, Jianlin Zhao

Author Affiliations +

Optical Engineering, Vol. 56, Issue 11, 111712 (July 2017). https://doi.org/10.1117/1.OE.56.11.111712

Abstract

Biological cells are usually transparent with a small refractive index gradient. Digital holographic interferometry can be used in the measurement of biological cells. We propose a dual-wavelength common-path digital holographic microscopy for the quantitative phase imaging of biological cells. In the proposed configuration, a parallel glass plate is inserted in the light path to create the lateral shearing, and two lasers with different wavelengths are used as the light source to form the dual-wavelength composite digital hologram. The information of biological cells for different wavelengths is separated and extracted in the Fourier domain of the hologram, and then combined to a shorter wavelength in the measurement process. This method could improve the system’s temporal stability and reduce speckle noises simultaneously. Mouse osteoblastic cells and peony pollens are measured to show the feasibility of this method.

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Jianglei Di, Yu Song, Teli Xi, Jiwei Zhang, Ying Li, Chaojie Ma, Kaiqiang Wang, and Jianlin Zhao "Dual-wavelength common-path digital holographic microscopy for quantitative phase imaging of biological cells," Optical Engineering 56(11), 111712 (11 July 2017). https://doi.org/10.1117/1.OE.56.11.111712

Received: 15 April 2017; Accepted: 15 June 2017; Published: 11 July 2017

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