2 April 2020 Pulsed illumination phase-contrast microscopy
Kevin J. Ly, Sy-Bor Wen
Author Affiliations +
Abstract

With high spatial but low temporal coherent nanosecond (ns) green light, ns pulsed illumination phase-contrast microscopy is successfully constructed. The pulsed illumination phase-contrast microscopy can provide ns time resolution to resolve transient dynamics of transparent targets with high contrast ratio that cannot be achieved with traditional bright-field or phase-contrast microscopies. Transient dynamics of laser-induced microcavitation is successfully recorded with this imaging technique to demonstrate its capabilities. In addition to tracing the evolutions of vapor bubbles and shockwaves during a laser-induced microcavitation, this imaging technique can sense the refractive index change of the transparent target during a pressure field variation.

© 2020 Society of Photo-Optical Instrumentation Engineers (SPIE) 0091-3286/2020/$28.00 © 2020 SPIE
Kevin J. Ly and Sy-Bor Wen "Pulsed illumination phase-contrast microscopy," Optical Engineering 59(4), 043101 (2 April 2020). https://doi.org/10.1117/1.OE.59.4.043101
Received: 15 December 2019; Accepted: 20 March 2020; Published: 2 April 2020
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KEYWORDS
Microscopy

Pulsed laser operation

Objectives

Refractive index

Optical fibers

Water

Light

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