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4 May 2020 Dark-field illumination in conjunction with confocal Raman spectroscopy for real-time noninvasive aqueous humor investigation
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Abstract

To obtain a real-time noninvasive analysis of the content of the aqueous humor (AH) in the eye, a confocal dark-field ophthalmic probe was designed and optimized by ray-tracing software. The confocal technique allows Raman spectrometric sampling of the AH with suppressing signals from the surrounding tissues. Dark-field illumination prevents the excitation light to reach the vulnerable retinal tissue directly. In order to evaluate the optical performance and safety of this method, a functional prototyped eye contact probe has been designed and tested on ex vivo rabbit eyes.

© 2020 Society of Photo-Optical Instrumentation Engineers (SPIE) 0091-3286/2020/$28.00 © 2020 SPIE
Shuo Zhang, Roel J. Erckens, Franciscus H. M. Jongsma, John De Brabander, Carroll A. B. Webers, and Tos T. J. M. Berendschot "Dark-field illumination in conjunction with confocal Raman spectroscopy for real-time noninvasive aqueous humor investigation," Optical Engineering 59(9), 092002 (4 May 2020). https://doi.org/10.1117/1.OE.59.9.092002
Received: 13 December 2019; Accepted: 27 March 2020; Published: 4 May 2020
JOURNAL ARTICLE
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