We have investigated the multi-spectral line output characteristics of a CO₂ pulsed laser at multi-atmospheric pressure. The pulse waveforms, wavelengths, and energies of the output spectral lines and the small-signal gains of the full spectrum were measured at 3, 4, and 6 atm. To explain the experimental results and predict the output at higher pressure, a modified six-temperature multi-frequency dynamic model of the regular and sequence band lines was developed. The output spectrum varying from the 10P to 9R band with the increase of pressure was studied theoretically and experimentally. In addition, the gains of the 9R(16), 9P(16), 10R(16), and 10P(16) lines at 3 to 14 atm were simulated and measured, providing insights into variation regulation of the four band gains as pressure increases.