This paper presents an optical approach to estimate the thickness of biofilms on metals in natural aqueous environment.
An in-situ fibre optic sensor is held above the sample surface, to offer real time information continuously by measuring
the spatially resolved profiles of scattered light in biofilms. The optical results correlate well with the biofilm thickness
which is measured using a microscope and a microcapillary with a protruding thin, long flexible wire from the top of the
microcapillary. The biofilm thickness is less than 120 &mgr;m on test material after 20 days. Microscopic image analysis also
provides the evidence to confirm micro colony formation by rod and cocci bacterial species. The species Klebsiella are
found to be dominant on carbon steel. It is observed that measured optical intensity is proportional to the concentration
of organisms.
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