A DNA-CTMA optical fiber was prepared by melt-spinning method for the first time. A hemicyanine dye, trans-4-(4-dibutylamino)-styryl)-1-methylpyridinium iodide (DBASMPI) doped DNA-CTMA fiber with core diameter of 1 mm and dye concentration of 3.6 wt% was obtained by soaking it in an aqueous dye solution. Laser (532 nm) pumped amplified spontaneous emission (ASE) at 610 nm was observed in the dye-doped DNA-CTMA fiber. The ASE occured at energy density 50 mW. The results from ASE emphasize that DBASMPI doped DNA-CTMA fiber is appealing as a good candidate for optical amplifiers and superfluorescence sources in a variety of communication and sensor applications.