The article presents the results of algorithmic approbation of digital histological research methods of the age of damage to the tissues of the brain, liver and kidney, as well as the myocardium and lung tissue on the basis of polarization reconstruction of linear birefringence maps of protein fluorophores of fibrillar networks; determining the relationship between the temporal change in the magnitude of the statistical moments of the 1st - 4th orders, characterizing the distribution of the magnitude of the degree of crystallization of histological sections of the brain, liver and kidney, as well as the myocardium and lung tissue, and the duration of damage; establishment of time intervals and accuracy of determining the duration of damage to human internal organs by digital histological methods of polarization reconstruction (tomography) the degree of crystallization of samples of histological sections of the brain, liver and kidney, as well as myocardium and lung tissue.
The article contains the results of experimental testing of methods of azimuthal-invariant Mueller-matrix microscopy (Mueller-matrix invariants - MMI) of optically anisotropic fluorophores of samples of histological sections of the brain, liver and kidney, as well as myocardium and lung tissue; temporal detection of variations in the magnitude of the statistical moments of the 1st - 4th orders, characterizing the distributions of the MMI value of linear birefringence and optical activity of samples of histological sections of the brain, liver and kidney, as well as myocardium and lung tissue with different age of damage; determination of the diagnostic efficiency (time interval and accuracy) of establishing the age of damage to human internal organs by digital histological methods of MMI mapping of optical anisotropy of fluorophores in histological sections of the brain, liver and kidney, as well as myocardium and lung tissue.
The paper presents the results of experimental testing of methods for azimuthal-invariant polarization mapping of laser induced microscopic images of fluorophores in histological sections of the liver of deceased; time monitoring of changes in the magnitude of statistical moments of the 1st - 4th orders characterizing the distributions of the azimuth and ellipticity of polarization of microscopic images of histological sections of the liver with different age of damage; determination of the diagnostic efficiency (time interval and accuracy) of establishing the age of damage to human internal organs by digital histological methods of mapping maps of azimuth and ellipticity of polarization of microscopic images of samples of histological sections of the brain, liver and kidney, as well as myocardium and lung tissue.
The results of algorithmic testing of digital histological studies of the duration of kidney tissue damage based on polarization reconstruction of linear birefringence maps of protein fibrillar networks are presented. The relationships between the temporary change in the magnitude of the statistical moments of the 1st - 4th orders of magnitude characterizing the distribution of the degree of crystallization of histological sections of the kidney and the duration of damage are determined.
By Mueller-matrix mapping of changes in the distribution of the magnitude of matrix invariants characterizing the degree of crystallization of histological sections of the brain, liver and kidney, the forensic medical criteria were determined and the interval for determining the damage duration of 24 hours with an accuracy of 45min. - 50 min. was established. For azimuthally invariant polarization mapping of changes in the distributions of Mueller-matrix invariants characterizing the optical activity of molecular complexes of histological sections of the brain, liver, and kidney, forensic medical criteria were developed for the first time and accuracy was improved to 35 min. - 40 min. in the interval for determining the damage duration period of 72 hours. For highly accurate objective determination of the damage duration in a long time interval (1 hour - 120 hours), a polarization tomography method has been developed (reproducing the distributions of birefringence of fibrillar networks of histological sections of internal organs), which provides an accuracy of 25 minutes. (1 hour - 24 hours) to 45 minutes (24 hours - 120 hours).
For a high-precision objective histological determination of the prescription of damage to internal organs over a long period of time, a systematic approach was used based on digital azimuthally invariant polarizing, Mueller-matrix and tomographic methods for studying temporary changes in the molecular and polycrystalline structure of brain, liver and kidney samples in the post-mortal period. It was revealed that a linear change in the magnitude of statistical moments of the 1st - 4th orders characterizing the distribution of data of digital azimuthally invariant polarizing, Mueller-matrix and tomographic methods is interconnected with the duration of damage to internal organs. On this basis, a new algorithm for digital histological determination of the prescription of the occurrence of damage is proposed. To determine the extent of damage, the method of azimuthally invariant polarization microscopy with different magnification of the image of histological sections of tissues of internal organs was used, which provided diagnostic relationships between changes in the magnitude of statistical moments of the 1st to 4th orders, which characterize the azimuth and elliptic polarization maps of digital microscopic images and time intervals of damage duration.
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