During glioblastoma surgery, delineation of the brain tumour margins remains difficult especially since
infiltrated and normal tissues have the same visual appearance. This problematic constitutes our research
interest. We developed a fibre-optical fluorescence probe for spectroscopic and time domain measurements.
First measurements of endogenous tissue fluorescence were performed on fresh and fixed rat tumour brain
slices. Spectral characteristics, fluorescence redox ratios and fluorescence lifetime measurements were analysed.
Fluorescence information collected from both, lifetime and spectroscopic experiments, appeared promising for
tumour tissue discrimination. Two photon measurements were performed on the same fixed tissue. Different
wavelengths are used to acquire two-photon excitation-fluorescence of tumorous and healthy sites.