The hardware-based Anti-Brownian ELectrokinetic trap (ABEL trap) features a feedback latency as short as 25 μs,
suitable for trapping single protein molecules in aqueous solution. The performance of the feedback control loop is
analyzed to extract estimates of the position variance for various controller designs. Preliminary data are presented in
which the trap is applied to the problem of determining the distribution of numbers of ATP bound for single chaperonin
multi-subunit enzymes.
The Anti-Brownian Electrophoretic trap (ABEL trap) allows a user to trap and manipulate individual fluorescent molecules in solution. The heart of the ABEL trap is a microfluidic cell. In previous incarnations of the ABEL trap, the microfluidic cell was formed from a polydimethylsiloxane (PDMS) stamp and a glass coverslip. Here we present an improved microfluidic cell, made entirely out of glass. This new design significantly decreases the rate of photobleaching, which previously limited the time that a single molecule could be trapped. Chemical modifications to the surface of the cell prevent adsorption and allow one to control the balance between electroosmotic and electrophoretic forces. The depth of the trapping region in the cell can be adjusted to allow trapping of different-sized objects.
The Anti-Brownian ELectrophoretic trap (ABEL trap) is a new device that allows a user to trap and manipulate fluorescent objects as small as 20 nm freely diffusing in solution. We describe in detail how to build an ABEL trap.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.