This paper presents the synthesis of gold nanoparticles (AuNPs) suitable to the construction of engineering biosensors. The phenomenon of Localized Surface Plasmons Resonance (LSPR) and Surface Plasmon Polarization are explored in these sensors. AuNPs allow developing nanoscale devices that interacts with chemical and biological systems. The LSPR is the main operation principle explored in these nanosystems that promote electromagnetic wave oscillation on such small metallic structures. Our results have shown that the resonance is directly linked to the size of the nanoparticles, the nature of the dielectric material and support environment where the device is being studied, previously reported for Zhao et al. . Nanoparticles with size of 56 to 88 nm was obtained this work. The Turkevich method was to used to make AuNP’s with spherical morphology, this system is it possible to application in biosensor because your strong interation with light and environment.
Studies to determine the concentration of hydrogen peroxide (H2O2) are important in biological system due to cellular damages provoked by reative oxygen species that include H2O2. An alternative to detect H2O2 is through an optical nanosensors based on silver nanoparticles, which have great potential for chemical and biological sensing applications. Here we demonstrate that attenuated total reflectance (ATR) from interaction of silver nanoparticles and hydrogen peroxide were able to detect very low levels of H2O2 around 0,001mM.