Raman spectroscopy has become an essential characterization tool for chemical and biomedicine, capable of single-cell sensitivity with high spatial resolution. We applicate single-cell Raman microspectroscopy (SCRS) into cancer cells and aim to investigate the practical distribution of glycogen in the brain cancer cell and analyze the main pathway, moreover, try to give an insight of how glycogen metabolism affects Krebs circle, cancer metastasis and invasion. Here, we found the high glycogen points are more likely to concentrate at the cytoplasm randomly, and the glycogen concentration of cancer cell grown under stress condition is higher than that of normal condition.
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