A fundamental task in studying the action of cancer chemotherapy is to determine the quantity and spatial relationship of tumor-infiltrating lymphocyte populations. Classically this is performed by staining thin tissue sections with antibodies by immunoperoxidase amplification. The staining technique is practically limited to locating a single cell type per tissue section. Full immunophenotyping requires successive staining of serial sections, using statistical analysis to correlate the results. This paper describes a system that brings together multi- parameter fluorescence imaging and morphological segmentation techniques to provide a fast, accurate, and automatic analysis of the lymphocyte infiltrate in tissue sections. With fluorescence techniques a single section can be stained with up to four distinct fluorescently labelled antibodies to determine cell phenotypes. To harness this potential computer vision techniques are required to analyze the images. A routine based on the water shed algorithm has been developed that segments the nuclei image with an accuracy of greater than 90%. By matching the nuclei boundaries to the local peak fluorescence, cell boundary estimates are obtained in the antigen images. By then extracting two measurements from the boundary signal the cells can be classified according to their antigen expression. Determining cell expression of multiple antigens simultaneously provides a more detailed and accurate picture of the tumor infiltrate than single parameter analysis, and increases understanding of the immune response associated with the chemotherapy.
Conference Committee Involvement (7)
Small Animal Whole-Body Optical Imaging Based on Genetically Engineered Probes
23 January 2007 | San Jose, California, United States
Genetically Engineered Probes for Biomedical Applications
24 January 2006 | San Jose, California, United States
Genetically Engineered and Optical Probes for Biomedical Applications
25 January 2005 | San Jose, CA, United States
Genetically Engineered Fluorescent and Bioluminescent Probes
24 January 2004 | San Jose, CA, United States
Manipulation and Analysis of Biomolecules, Cells and Tissues
28 January 2003 | San Jose, CA, United States
Genetically Engineered Probes
27 January 2003 | San Jose, CA, United States
Functional Imaging and Optical Manipulation of Living Cells and Tissues
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