Quantitative phase imaging (QPI) can be considered as a potential tool to extract important information on the refractive index of the cellular and subcellular structures. Interphase chromatin is an original biosensor, a detector of early changes in morphofunctional cell condition. The authors presented a technology of densitometric segmentation based on the quantitative phase microscopy and computed analysis of the changes in the optic density of interphase chromatin as a biosensor. The purpose of this work to evaluate the possibility of quantitative phase imaging for visualizing the nuclear proteins conformation and chromatin decondensation degree. Modification of chromatin structure, compactness of its package, and so on, are indicative of cell condition alteration and may be projected on the organism as a whole not only for the early preclinical diagnostics but also for assessment of prognosis in crisis conditions.
The objective of the present study is to increase the quality of the early diagnosis using cytological differential-diagnostic criteria for reactive changes in the nuclear structures of the immunocompetent cells. The morphofunctional status of living cells were estimated in the real time using new technologic platform of the hardware-software complex for phase cell imaging. The level of functional activity for lymphocyte subpopulations was determined on the base of modification of nuclear structures and decreasing of nuclear phase thickness. The dynamics of nuclear parameters was used as the quantitative measuring for cell activating level and increasing of proliferative potential.