Mr. Alexandr E. Polozhenkov Profile

Alexandr E. Polozhenkov

at Saratov State Medical Univ

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Proceedings Article | 29 April 2022 Paper

Morphological changes in rat lung tissue during inhalation of e-cigarette liquid aerosol

Alla Bucharskaya, Dmitry Mudrak, Irina Yanina, Nikita Navolokin, Alexandr Polozhenkov, Elina Genina, Yury Kistenev, Valery Tuchin

Proceedings Volume 12192, 1219210 (2022) https://doi.org/10.1117/12.2627915

KEYWORDS: Lung, Tissues, Liquids, Tissue optics, In vivo imaging, Optical coherence tomography, Scattering, Aerosols, Light scattering, Absorption

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For potential use as a diagnostic test, morphological changes in the lung tissue of rats after inhalationof e-cigarette liquid based on polypropylene glycol and glycerol were studied ex vivo and in vivo. Lungs of Wistar rats placed for 1 hour in ecigarette liquid were used in the ex vivo study. The in vivo study was performed on 12 mature male Wistar rats. The animals were divided into two groups - experimental and control, 6 rats in each. Rats of the experimental group were placed in aerosol administration chamber and exposed to an aerosol of nicotine-free liquid for electronic cigarettes. Rats of the control group were intact. The animals were removed from the experiment one hour after inhalation, lung tissue samples were examined ex vivo using optical coherence tomography (OCT) and lung tissue spectroscopy. After manipulations, lung samples were fixed in 10% formalin solution and embedded in paraffin. Then histological sections were made according to the conventional methods, followed by hematoxylin and eosin staining. Comparative analysis of B-scans ex vivo of lung tissue samples placed in e-cigarette liquid and control samples showed the presence of a luminescent effect on lung tissue in the studied solution, as alveoli are filled with the solution and scattering is reduced. The structure of the tissue differs from the control samples. B-scan in vivo studies are characterized by the presence of voids of rather large size. Histological examination of ex vivo and in vivo specimens noted morphological changes in lung tissue structure corresponding to the changes observed in OCT.

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