We describe the novel method of pbICS that enables us to determine the complex aggregation distributions of molecules. The method is applied to determine the clustering status of the EGF receptor on the surface of a CHO cell.
Finite-difference time-domain (FDTD) computational phantoms aid the analysis of THz radiation interaction with human skin. The presented computational phantoms have accurate anatomical layering and electromagnetic properties. A novel “large sheet” simulation technique is used allowing for a realistic representation of lateral absorption and reflection of in-vivo measurements. Simulations carried out to date have indicated that hair follicles act as THz propagation channels and confirms the possible role of melanin, both in nevi and skin pigmentation, to act as a significant absorber of THz radiation. A novel freezing technique has promise in increasing the depth of skin penetration of THz radiation to aid diagnostic imaging.
In our course of Biomedical Imaging, we introduced a research project as an assignment that included an online poster presentation. To assess the assignment, an adjusted criteria sheet was created, where it facilitated providing students with an effective feedback linked to particular criteria. Students are expected to produce a scientific poster to present the result of their investigation and upload it to an online discussion board. In addition, they are required to read their colleagues’ works and provide peer-feedback by asking quality questions about principles and results, also on-line. Subtle distribution of marks in the rubric balances focus between preparing poster and providing peer-feedbacks.
Recently, new types of silica polarization converters fabricated by femtosecond lasers have been introduced. These devices use spatially arranged nanogratings found under certain femtosecond laser exposure conditions in fused silica to create arbitrary polarization states by shaping spatially and locally the retardance of an incoming beam. Using this principle, radial and azimuthal polarization converters were demonstrated. These devices make use of a large density of femtosecond laser spots, introducing localized defects, affecting the performance of the converter. To optimize the writing and the post-processing annealing step of these kind of devices, here we introduce a novel fluorescence lifetime imaging microscope (FLIM) working with deep UV (240-280 nm) wavelength excitations. Specifically, we demonstrate the potential of this technique and more generally, how it can be used for characterizing a variety of femtosecond laser induced modifications in fused silica. This UV-FLIM can be used with micro-fluidic and bio-samples to characterize temporal characteristics of fluorescence.