The development of a chemical sensor based on steering-wheel photonic crystal fiber (SW-PCF) and a NanoSpectrometerTM from Nano-Optics Devices, LLC can benefit industrial process-monitoring and environmental sensing applications. This chemical sensor can potentially result in a compact, image-based sensor with enhanced spectral resolution (~0.15nm) for applications such as environmental monitoring of water quality or quality control of pharmaceutical production. A nanospectrometer is a planar spectrometer-on-chip that can be combined with a number of light sources. The chip diffracts incident light to a series of wavelength dependent spatially addressed units that can be imaged and collected with a CCD camera. It is compact in size (10 mm × 15 mm × 0.5 mm) and has a high spectral resolution of 2×10-5um. This study is an extension of a previous investigation of water-filled SW-PCF spectroscopy. Instead of analyzing water samples fluorescent dyes were tested. Different types of dyes that absorbed and emitted light in the same spectral window as the chip were identified. Spectroscopy measurements for nile blue perchlorate dye are presented in this conference paper. A 70 mW laser at 637nm was employed to demonstrate the fluorescence spectroscopy capability of SW-PCF enhanced spectroscopy with a nanospectrometer. It was demonstrated that the SW-PCF is suitable for spectroscopy of dyes with a conventional optical spectrum analyzer and a nanospectrometer. A 5 microliter sample of dye was loaded into a 14cm long SW-PCF. The fluorescence-spectroscopic data was compared to an un-filled SW-PCF. Absorption and emission spectra for the dye were measured near 637nm.