Prof. Ammasi Periasamy
Professor/Center Director at Univ of Virginia
SPIE Involvement:
Conference Program Committee | Conference Chair | Track Chair | Editor | Author | Instructor
Area of Expertise:
Light Microscopy techniques , FLIM , FRET , Endogenous molecular imaging , NADH, FAD, Tryptophan , In Vivo/Intravital imaging
Profile Summary

A key area of my research is focused on the design and development of optical methodology including advanced light microscopy techniques to investigate/monitor exogenous and endogenous protein-protein interactions, in vivo/intravital imaging and monitoring the physical parameters of normal versus cancer tissues. I am the founder and center director of the W.M. Keck Center for Cellular Imaging (KCCI), an internationally known center in advanced optical and microscopy imaging, particularly in the area of protein-protein interactions in living and fixed specimens. We developed a 2- and 3-color steady state, and FLIM based Förster resonance energy transfer (FRET) imaging system for protein localization in living specimens. We developed a PFRET software package for quantitative FRET data analysis to estimate the nanometer (1-10 nm) distance between the protein molecules in living/fixed cells and tissue for various light microscopy techniques for any combination of fluorophore pairs. Recently, we developed FLIRR method to estimate the metabolic redox ratio in tissue, animal and cells. I am one of the pioneers in developing lifetime imaging microscopy for intracellular calcium measurement and later I developed the same methodology for protein-protein interactions and cancer diagnosis. I have published over 135 articles in refereed journals and book chapters. I have given more than 150 invited lectures nationally and internationally. I have edited three books, Chairperson in organizing an annual International conference on Multiphoton Microscopy in the Biomedical Sciences through SPIE (since 2001) and runs a hands on training annual workshop on FRET Microscopy at the University of Virginia, Charlottesville during March (since 2002). I was elected “Fellow” members of the SPIE.
Publications (71)

Proceedings Article | 13 March 2024 Presentation
Proceedings Volume PC12847, PC1284701 (2024)

Proceedings Article | 12 March 2024 Poster + Paper
Proceedings Volume 12847, 128470Q (2024)
KEYWORDS: Fluorescence lifetime imaging, Animals, Image processing, Mitochondria, In vivo imaging, Alzheimer disease, Cranial windows, Brain

Proceedings Article | 25 April 2023 Poster + Paper
Brian Mbogo, Karsten Siller, Jiaxin Zhang, Horst Wallrabe, Shagufta Rehman Alam, Ammasi Periasamy
Proceedings Volume 12384, 123840Q (2023)
KEYWORDS: Machine learning, Fluorescence lifetime imaging, Feature extraction, Data modeling, Image analysis, Artificial neural networks, Time correlated single photon counting

Proceedings Article | 15 March 2023 Presentation
Proceedings Volume PC12384, PC123840L (2023)

Proceedings Article | 4 March 2022 Presentation + Paper
Proceedings Volume 11965, 119650B (2022)
KEYWORDS: Fluorescence lifetime imaging, Cell death, Super resolution microscopy, Mode conditioning cables, Luminescence, Imaging systems, Signal attenuation, Cancer, Laser induced damage, Pulsed laser operation

Showing 5 of 71 publications
Proceedings Volume Editor (25)

SPIE Conference Volume | 4 April 2024

SPIE Conference Volume | 25 April 2023

SPIE Conference Volume | 11 April 2022

SPIE Conference Volume | 16 April 2021

SPIE Conference Volume | 5 March 2020

Showing 5 of 25 publications
Conference Committee Involvement (44)
Multiphoton Microscopy in the Biomedical Sciences XXV
25 January 2025 | San Francisco, California, United States
Advanced Chemical Microscopy for Life Science and Translational Medicine 2025
25 January 2025 | San Francisco, California, United States
Multiphoton Microscopy in the Biomedical Sciences XXIV
28 January 2024 | San Francisco, California, United States
Advanced Chemical Microscopy for Life Science and Translational Medicine 2024
27 January 2024 | San Francisco, California, United States
Multiphoton Microscopy in the Biomedical Sciences XXIII
29 January 2023 | San Francisco, California, United States
Showing 5 of 44 Conference Committees
Course Instructor
SC819: Multiphoton Microscopy (MM) - Basics, Technology Development, and Applications
This course introduces the basics of multiphoton microscopy, technology development, image acquisition and analysis related to various biological and clinical applications. The first part of the course will cover the basics of microscopy, image formation, detectors, lenses used in MM, and calculation of absorption cross-sections. The second part of the course will focus on how to build your own MM, or integrate one with an existing confocal microscope. The tricks involved in MM development and imaging will be covered. Finally, we will review many applications including tissue imaging, MM in Neuro and developmental biology, labeling issues for MM imaging, FLIM, FRET and other clinical applications.
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