Prof. Ammasi Periasamy
Professor/Center Director at Univ of Virginia
SPIE Involvement:
Fellow status | Conference Chair | Conference Program Committee | Track Chair | Journal Editorial Board Member | Author | Editor | Instructor
Area of Expertise:
Light Microscopy techniques , FLIM , FRET , Endogenous molecular imaging , Intravital imaging
Websites:
Profile Summary

A key area of my research is focused on the design and development of optical methodology including advanced light microscopy techniques to investigate/monitor exogenous and endogenous protein-protein interactions, intravital imaging and monitoring the physical parameters of normal versus cancer tissues. I am the founder and center director of the W.M. Keck Center for Cellualr Imaging (KCCI), an internationally known center in advanced optical and microscopy imaging, particularly in the area of protein-protein interactions in living and fixed specimens. We developed a 2- and 3-color steady state, confocal, multiphoton, and FLIM based Förster resonance energy transfer (FRET) imaging system for protein localization in living specimens. The important aspect of the FRET-work is the development of a PFRET software package for quantitative FRET data analysis to estimate the nanometer (1-10 nm) distance between the protein molecules in living/fixed cells and tissue for various light microscopy techniques for any combination of fluorophore pairs. This PFRET software is used nationally and internationally by the FRET microscopy users. I am an internationally recognized expert in advanced microscopy techniques, particularly in the area of molecular imaging in living cells and tissues. I am one of the pioneers in developing lifetime imaging microscopy for intracellular calcium measurement and later I developed the same methodology for protein-protein interactions and cancer diagnosis. I have published over 100 articles in refereed journals and book chapters. I have given more than 100 invited lectures nationally and internationally. I have edited three books, Chairperson in organizing an annual International conference on Multiphoton Microscopy in the Biomedical Sciences through SPIE (since 2001) and runs a hands on training annual workshop on FRET Microscopy at the University of Virginia, Charlottesville during March (since 2002). I was elected “Fellow” members of the SPIE.
Publications (74)

PROCEEDINGS ARTICLE | July 28, 2017
Proc. SPIE. 10414, Advances in Microscopic Imaging
KEYWORDS: Microscopy, Molecules, Image analysis, Fluorescence lifetime imaging, Prostate cancer, Fluorescence resonance energy transfer

SPIE Conference Volume | May 17, 2017

PROCEEDINGS ARTICLE | March 2, 2017
Proc. SPIE. 10069, Multiphoton Microscopy in the Biomedical Sciences XVII
KEYWORDS: Microscopes, Photon counting, Multiphoton microscopy, Imaging systems, Sensors, Image processing, Microscopy, Luminescence, Fluorescence lifetime imaging, Mode conditioning cables

PROCEEDINGS ARTICLE | February 21, 2017
Proc. SPIE. 10069, Multiphoton Microscopy in the Biomedical Sciences XVII
KEYWORDS: Confocal microscopy, Microscopes, Principal component analysis, Oncology, Cancer, Tissues, Imaging systems, Microscopy, Luminescence, Photons, Quantitative analysis, Cell death, Fluorescence lifetime imaging, Prostate cancer, Mode conditioning cables

SPIE Conference Volume | May 18, 2016

PROCEEDINGS ARTICLE | March 14, 2016
Proc. SPIE. 9712, Multiphoton Microscopy in the Biomedical Sciences XVI
KEYWORDS: Confocal microscopy, Microscopes, Glucose, Multiphoton microscopy, Cancer, Tissues, Imaging systems, Microscopy, Prostate, Fluorescence lifetime imaging, Prostate cancer, Fluorescence resonance energy transfer

Showing 5 of 74 publications
Conference Committee Involvement (31)
Multiphoton Microscopy in the Biomedical Sciences XVIII
28 January 2018 | San Francisco, California, United States
Multiphoton Microscopy in the Biomedical Sciences XVII
29 January 2017 | San Francisco, California, United States
Multiphoton Microscopy in the Biomedical Sciences XVI
14 February 2016 | San Francisco, California, United States
Multiphoton Microscopy in the Biomedical Sciences XV
8 February 2015 | San Francisco, California, United States
Multiphoton Microscopy in the Biomedical Sciences XIV
2 February 2014 | San Francisco, California, United States
Showing 5 of 31 published special sections
Course Instructor
SC819: Multiphoton Microscopy (MM) - Basics, Technology Development, and Applications
This course introduces the basics of multiphoton microscopy, technology development, image acquisition and analysis related to various biological and clinical applications. The first part of the course will cover the basics of microscopy, image formation, detectors, lenses used in MM, and calculation of absorption cross-sections. The second part of the course will focus on how to build your own MM, or integrate one with an existing confocal microscope. The tricks involved in MM development and imaging will be covered. Finally, we will review many applications including tissue imaging, MM in Neuro and developmental biology, labeling issues for MM imaging, FLIM, FRET and other clinical applications.
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