Prof. Ammasi Periasamy
Professor/Center Director at Univ of Virginia
SPIE Involvement:
Fellow status | Conference Chair | Conference Program Committee | Track Chair | Journal Editorial Board Member | Author | Editor | Instructor
Area of Expertise:
Light Microscopy techniques , FLIM , FRET , Endogenous molecular imaging , NADH, FAD, Tryptophan , In Vivo/Intravital imaging
Profile Summary

A key area of my research is focused on the design and development of optical methodology including advanced light microscopy techniques to investigate/monitor exogenous and endogenous protein-protein interactions, in vivo/intravital imaging and monitoring the physical parameters of normal versus cancer tissues. I am the founder and center director of the W.M. Keck Center for Cellular Imaging (KCCI), an internationally known center in advanced optical and microscopy imaging, particularly in the area of protein-protein interactions in living and fixed specimens. We developed a 2- and 3-color steady state, and FLIM based Förster resonance energy transfer (FRET) imaging system for protein localization in living specimens. We developed a PFRET software package for quantitative FRET data analysis to estimate the nanometer (1-10 nm) distance between the protein molecules in living/fixed cells and tissue for various light microscopy techniques for any combination of fluorophore pairs. Recently, we developed FLIRR method to estimate the metabolic redox ratio in tissue, animal and cells. I am one of the pioneers in developing lifetime imaging microscopy for intracellular calcium measurement and later I developed the same methodology for protein-protein interactions and cancer diagnosis. I have published over 115 articles in refereed journals and book chapters. I have given more than 150 invited lectures nationally and internationally. I have edited three books, Chairperson in organizing an annual International conference on Multiphoton Microscopy in the Biomedical Sciences through SPIE (since 2001) and runs a hands on training annual workshop on FRET Microscopy at the University of Virginia, Charlottesville during March (since 2002). I was elected “Fellow” members of the SPIE.
Publications (81)

Proceedings Article | 29 March 2019
Proc. SPIE. 10873, Optical Biopsy XVII: Toward Real-Time Spectroscopic Imaging and Diagnosis
KEYWORDS: Multiphoton microscopy, Image segmentation, Microscopy, Molecules, Single photon, Fluorescence lifetime imaging, Prostate cancer, Molecular imaging, Signal analyzers, Data analysis

Proceedings Article | 27 March 2019
Proc. SPIE. 10882, Multiphoton Microscopy in the Biomedical Sciences XIX
KEYWORDS: Microfluidics, Medicine, Oncology, Cancer, Image segmentation, Microscopy, Luminescence, Fluorescence lifetime imaging, Leukemia, Auto-fluorescence imaging

Proceedings Article | 4 March 2019
Proc. SPIE. 10882, Multiphoton Microscopy in the Biomedical Sciences XIX
KEYWORDS: Multiphoton microscopy, Cancer, Tissues, Microscopy, Luminescence, Fluorescence lifetime imaging, Fluorescence resonance energy transfer, Molecular interactions, Cell mechanics, Mode conditioning cables

Proceedings Article | 4 March 2019
Proc. SPIE. 10881, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVII
KEYWORDS: Proteins, Cancer, Imaging systems, Microscopy, Luminescence, Molecules, Collagen, Fluorescence lifetime imaging, Prostate cancer, Signal detection

SPIE Conference Volume | 11 April 2018

Proceedings Article | 23 February 2018
Proc. SPIE. 10498, Multiphoton Microscopy in the Biomedical Sciences XVIII
KEYWORDS: Hyperspectral imaging, Near infrared, Microscopes, Visible radiation, Optical sensors, Biomedical optics, Tissues, Imaging systems, Video, Luminescence

Showing 5 of 81 publications
Conference Committee Involvement (34)
Advanced Chemical Microscopy for Life Science and Translational Medicine
1 February 2020 | San Francisco, California, United States
Multiphoton Microscopy in the Biomedical Sciences XX
1 February 2020 | San Francisco, California, United States
Multiphoton Microscopy in the Biomedical Sciences XIX
3 February 2019 | San Francisco, California, United States
Multiphoton Microscopy in the Biomedical Sciences XVIII
28 January 2018 | San Francisco, California, United States
Multiphoton Microscopy in the Biomedical Sciences XVII
29 January 2017 | San Francisco, California, United States
Showing 5 of 34 published special sections
Course Instructor
SC819: Multiphoton Microscopy (MM) - Basics, Technology Development, and Applications
This course introduces the basics of multiphoton microscopy, technology development, image acquisition and analysis related to various biological and clinical applications. The first part of the course will cover the basics of microscopy, image formation, detectors, lenses used in MM, and calculation of absorption cross-sections. The second part of the course will focus on how to build your own MM, or integrate one with an existing confocal microscope. The tricks involved in MM development and imaging will be covered. Finally, we will review many applications including tissue imaging, MM in Neuro and developmental biology, labeling issues for MM imaging, FLIM, FRET and other clinical applications.
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