Microscope-integrated optical coherence tomography (MIOCT) systems allow for 4D visualization of thin,
semi-transparent structures during ophthalmic microsurgery. While these visualizations have greatly
increased the information available to surgeons, new surgical techniques, such as subretinal injections of
gene therapies, would benefit from quantitative measurements of structures imaged using OCT. Calibration
of true scan dimensions in OCT is complicated by inter-patient ocular variability as well as poor control of
scanner-patient alignment. We introduce novel measurement techniques for calibration and de-warping of
OCT imagery which allows for accurate measurement of intraocular structures including subretinal
microinjection bleb volumes in the ocular posterior segment.