Imaging live biological samples to study biomolecular dynamics requires a very high spatial and temporal resolution.
Superresolution localization microscopy has allowed researchers to investigate biological systems whose sizes are below
the diffraction limit (200-250 nm) using an optical microscope. Fluorescence Photoactivation Localization Microscopy
(FPALM) and other localization microscopy techniques have recently been shown to be capable of rendering
superresolution images obtained with acquisitions of shorter than 0.5 seconds. Here we will discuss the FPALM imaging
technique, at both lower and higher imaging speeds. This talk will focus on the advantages, challenges, and drawbacks
of high speed imaging localization microscopy.