The structure of an artificial ligament was examined using Raman microscopy
in combination with novel data analysis. Basis approximation and compressed principal component
analysis are shown to provide efficient compression of confocal Raman microscopy
images, alongside powerful methods for unsupervised analysis. This scheme allows the acceleration
of data mining, such as principal component analysis, as they can be performed on
the compressed data representation, providing a decrease in the factorisation time of a single
image from five minutes to under a second.
Using this workflow the interface region between a chemically engineered ligament construct
and a bone-mimic anchor was examined. Natural ligament contains a striated interface
between the bone and tissue that provides improved mechanical load tolerance, a similar
interface was found in the ligament construct.