Modulation interference microscopy is one of the promising technologies for early personalized cancer diagnostics that allow for assessment of the real-time changes of subcellular microstructures with 2D and 3D reconstructions of the images and multifactorial data analysis. Our investigation is aimed to assess the heterogeneity of the CTCs population in patients with breast cancer using modulation interference microscopy (Quantitative Phase Imaging (QPI) technology). Morpho-functional changes in living CD326 (EpCam)+ and Annexin <sup>V</sup>+ cell were measured with a laser modulation interference microscope MIM (PA UOMZ, Russia): height accuracy 0,1 nm, coordinate accuracy 10 nm, image area 1280x1024 pixels, optical magnification 1000, acquisition time 0,3 sec. The complex algorithm included the definition of optic and geometrical characteristics of living cells and , statistical analysis of data and creation of medical documents. We evaluated the functional cellular conditions based on the phase-interference features of their nuclear structures including nucleoli organizer areas which reflect metabolic and proliferative activity of cells and also serve the markers of their malignant transformation.
Revealing and characterization of circulating tumor cells (CTCs) is one of the most actively investigated field of oncology. It was established by the past time studies that some of these cells were in condition of apoptosis and so couldn’t initiate formation of a clinically significant metastasis. The novel opportunities associated with development of computer technologies and interferential microscopes facilitate the solution of many medico-biological problems. One of them is a method of determination of CTCs functional condition based on the phase-interferential characteristics of their nuclear structures which reflect cellular metabolic and proliferative activity and are the markers of their malignant transformation. <p> </p>The analysis was carried out of the morphodensitometric biomarkers of the metastatic activity of circulating tumorous cells including conformational alterations of their nuclear structures. The reference collection of phase images of metastatic cells was created as well as the data of the morphodensitometric parameters of their metabolic activity was received. <p> </p>A new information was received about heterogeneity of the circulating tumorous cells with different metastatic activity which allows the broadening of fundamental scientific knowledge concerning cancerogenesis, individualization of the observation and treatment of patients, improvement of the quality of treatment, and optimization of its cost.