The effect of the laser pulse energy and total expose of the energy incident on the embryo blastomere fusion probability was investigated. The probability of the four different events after laser pulse was determined: the fusion of two blastomeres with the following formation of tetraploid embryo, the destruction of the first blastomere occurs, the second blastomere conservation remains intact, the destruction and the death of both cells; two blastomeres were not fused, and no morphological changes occurred. We report on viability and quality of the embryo after laser surgery as a function of the laser energy incident. To characterize embryo quality, the probability of the blastocyst stage achievement was estimated and the blastocyst cells number was calculated. Blastocoel formation is the only event of morphogenesis in the preimplantation development of mammals, so we assumed it as an indicator of the time of embryonic “clocks” and observed it among fused and control embryos. The blastocoel formation time is the same for fused and control embryos. It indicates that embryo clocks were not affected due to blastomere fusion. Thus, the analysis of the fluorescence microscopic images of nuclei in the fused embryo revealed that nuclei fusion does not occur after blastomere fusion.
Vibrational wave packets in chloroform and gold colloid in chloroform were studied by femtosecond pump-probe
spectroscopy. It was found that the main modes of oscillations for both systems coincide with the frequencies of the
Raman resonances of chloroform 260 cm<sup>-1</sup>, 367 cm<sup>-1</sup> and 668 cm<sup>-1</sup>. The effect of gold nanoparticles leads to 4-6 fold
increased signal of Raman resonance of pure chloroform.