Laser-Induced-Autofluorescence (LIAF), White-Light-Remission (WLR) and Electrical-Impedance-Measurement (EIM)
in combination with principal component analysis were used at first time for differentiation benign from malignant
prostatic tissue in vitro. The used complex statistical processing of the data has shown a differentiation of benign and
malignant prostatic tissue with a sensitivity of 93.8% and a specificity of 92.4%.
We have investigated structural changes of human erythrocyte membranes under the action of interference light fields with different periods of spatial modulation. A local polarity in the surface regions of membranes was detected by means of the fluorescent probe method using stationary and time-resolved fluorescence spectroscopy. The electro-dipolar molecules 4'-diethylamino (FET) and 4'-<i>N</i>-(15-azacrown-5) (FCR) derivatives of 3-hydroxyflavone with coupled intramolecular charge and proton transfers were used as fluorescent probes. The FET molecules locate deeper in the membrane than FCR probes. It was established that the micropolarity of probes binding sites in membranes is changed after irradiation by the interference laser field. The largest effect was observed when erythrocytes were irradiated by the field with 7.2 μm period, which corresponds to maximal energy of interaction of an erythrocyte with a gradient light
To investigate of 4'-diethylamino (FET) and 4'-(15-azacrown- 5) (FCR) derivatives of 3-hydroxyflavone in binary solvents and erythrocyte ghosts, we used the red-edge excitation spectroscopy. The results obtained prove the existence of spectral heterogeneity of flavonols in the studied systems. The effect manifests itself in the dependence of the efficiency of excited-state intramolecular proton transfer on the excitation frequency.