Laser speckle contrast imaging (LSCI) is a simple and quite powerful method for visualization of flow, microcirculation and perfusion. In current study the speckle contrast variations towards breaking ergodicity conditions are considered with a final aim of envision a practical approach allowing real-time imaging of variations in dynamic properties of complex fluids with an opportunity of quantitative interpretation of the obtained flowing map. As example of systems with static to dynamic transition, melting of Intralipid samples were studied. Also, investigation of influence of static layer thickness above the dynamic sample on the ergodicity condition has been studied.
We report the results on in ovo application of developed Laser Doppler Anemometer (LDA) device. The chorioallantoic membrane (CAM) of 9-13 days chicken embryos was used as a biological model that allows an easy access to both arterial and venous vessels of different size. The key point of our study was to find out how the periodic and aperiodic pulsations of blood flow (which are inevitable in living organism) will affect the LDA functions and measuring capability. Specifically, we (i) developed the technique to extract and refine the pulse rhythm from the signal received from a vessel, and (ii) analyzed the changes in power spectra of LDA signal that are caused by heart beating and considerably complicate the reliable measurement of Doppler shift. Our main conclusion is that the algorithm of LDA data processing need to be improved, and this possibly can be done by counting the information on current phase of cardiac cycle.
Particularities of interference signal shaping in white-light interferometer with uncompensated dispersive layer are discussed. We especially attended to dependence of interference pulse position on the dispersive layer properties. Phase refractive index of the layer tends to be substantially nonlinear function of wavelength within the wide emission band of ultra-low coherence thermal light source. In this case, it is the group refractive index dispersion that is beginning to exert an influence on interference signal formation. It is shown experimentally that influence consists in nonlinear dependence of interference pulse position on geometrical thickness of the dispersive layer. The results show that mismatch of the dispersive layer and compensator refractive indices in the third place can produce interference signal shift on the order of pulse width.