Localization based super resolution images of a biological sample is generally achieved by using high power laser illumination with long exposure time which unfortunately increases photo-toxicity of a sample, making super resolution microscopy, in general, incompatible with live cell imaging. Furthermore, the limitation of photobleaching reduces the ability to acquire time lapse images of live biological cells using fluorescence microscopy. Digital Light Processing (DLP) technology can deliver light at grey scale levels by flickering digital micromirrors at around 290 Hz enabling highly controlled power delivery to samples. In this work, Digital Micromirror Device (DMD) is implemented in an inverse Schiefspiegler telescope setup to control the power and pattern of illumination for super resolution microscopy. We can achieve spatial and temporal patterning of illumination by controlling the DMD pixel by pixel. The DMD allows us to control the power and spatial extent of the laser illumination. We have used this to show that we can reduce the power delivered to the sample to allow for longer time imaging in one area while achieving sub-diffraction STORM imaging in another using higher power densities.
A thin film preparation technique leading to reduced polaron formation in thin films of the polymer poly(m-phenylenevinylene-co-2,5-dioctyloxy-p-phenylenevinylene) (PmPV) was used to prepare thin films with significantly improved photoluminescence efficiency. This was achieved through increased interchain separation in films prepared using this technique. Photoinduced absorption measurements were performed to study the nature of the increased photoluminescence efficiency. The electrical transport properties of PmPV films prepared using this preparation technique were measured using both direct and alternating current measurement techniques and found to be improved for positive charge carriers and unchanged for negative charge carriers relative to conventional preparation techniques. The relative permittivity was shown to be greater in this film type, due to the longer delocalisation lengths resulting from increased interchain separation in these films. Fabrication of single layer light emitting devices utilising PmPV prepared using this technique were found to be significantly brighter and to have longer device operating lifetimes.