We have developed a compact optical endoscopic probe for in vivo fluorescence optical imaging device. We obtained fluorescence image for the colon tissue of a mouse using a compacted optical endoscopic probe with a designed endoscopic optical lens. In order to demonstrate endoscopic fluorescence imaging for the colon cancer, we have manufactured a compacted optical endoscopic probe to pass through the biopsy channel of electric flexible endoscope. The compacted optical endoscopic probe with maximum outside diameter of 2.8mm consists of fiber-optic illumination part and imaging part. The imaging part consists of a fiber-optic imaging bundle linked to an endoscopic optical lens and focus assembly. We considered a compact structure, sensitivity, and FOV for the design of the endoscopic optical lens. We have suggested an endoscopic optical lens with an FOV of 90 ° and DOF of 3 – 80mm. The optical system consists of glass-based aspheric lenses. The total track is less than 2.5 mm, and the diameter is limited to less than 1.5 mm to obtain a compact system. We have presented the compacted optical endoscopic probe for cancer imaging of a mouse. The proposed endoscopic optical lens showed sufficient sensitivity and a wide field of view for obtaining fluorescence imaging. We demonstrated endoscopic ex vivo fluorescence imaging for the colon cancer of a mouse using the compacted optical endoscopic probe.
We have presented the plastic based ultra-compact aspheric lens for disposable epidural spinal endoscope. We have also showed the analysis of the stray light distribution on the image plane using optical illumination system design software (Light Tools). The optical system consists of the aspheric lens with a size of 1.4mm (total track of optical system). The effective length and field of view (FOV) is 0.66mm and 90 degrees. The distortion of the optical system is below 25%. The curves of modulation transfer function (MTF) are higher than 0.3 at 80 line pairs/mm (lps/mm) in image space. For the analysis of stray light, we assumed that the 98 percent of incident light is absorbed inside lens barrel and the rest is scattered on the inner surfaces of the lens barrel. The average value of stray light is 0.16% in the image intensity. The maximum stray light and minimum stray light of the proposed optical system is 0.57% and 0.0005% in the image intensity, respectively. The effective transmission rate of the proposed optical system is 89.6%.
KEYWORDS: Cameras, Endoscopes, Color reproduction, Endoscopy, Sensors, Light sources and illumination, Laparoscopy, In vivo imaging, Image display, Light sources, Xenon, Lamps, Medical imaging, RGB color model
Most of cameras like CCTV cameras or video cameras are shoot in available light or lightings. But the medical cameras like endoscope or laparoscopy are shoot in the situation in vivo. Generally, inside ad body is lightless and moist. So we can shoot the inside with a single light from outside the body. So medical cameras should be set more clearer and vivider in low-light than in high-light. The camera setting of disposable endoscopes is especially very important because those are low price and should be guaranteed against faulty workmanship. In this study, We suggest effective conditions for camera settings of disposable endoscopes, and request value of test measurement item with analysis from the commercial endoscopes.
We have studied the method to improve the illumination efficiency in DFE with optical illumination design software. We have showed the result of illumination efficiency according to the change of geometrical shape in distal tip of plastic optical fiber (POF). We simulated the illumination efficiency in the case of the polished POF distal tip and the unpolished one, respectively using optical illumination system design software. We obtained the illumination efficiency was increased by about 46 percent in the polished distal tip more than in the unpolished distal tip when a light emitting diode (LED) was directly excited to the distal tip of POF. In order to demonstrate the simulated results, we showed the polishing fabrication of the distal tip in POF and have measured the illumination efficiency of the polished POF using LED. The measured results showed that illumination efficiency was increased by about 23 percent in the polished distal tip more than in the unpolished distal tip of POF. We have demonstrated the optimized geometrical shapes of the POF for minimization of the illumination loss. We have suggested the method to improve the illumination efficiency by 69 percent for a single fiber illumination delivery system of DFE.
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