Mechanical forces and living cells are closely related, in particular in connections, named Focal Adhesions (FAs),
between cells and extracellular matrix. FAs are mechanosensors and regulate physiological processes.
The aim of this contribution is to explore the possibility of performing micrometric and submicrometric protein pattern
in order to study FAs on different materials. Typical substrates for microelectromechanical systems (MEMS) are tested
and the results are reported.
A laser is used to produce a pattern of extracellular matrix proteins, like fibronectin. A new use of a Raman microprobe
is described. Cells arrange in a regular shape, following the geometry of the pattern. Protein spots last for more than 24
It is very important to have a complete control on FAs and the technique proposed is a suitable solution.