Imaging Raman spectroscopy can be used to identify cancerous tissue. Traditionally, a step-by-step scanning of the sample is applied to generate a Raman image, which, however, is too slow for routine examination of patients. By transferring the technique of integral field spectroscopy (IFS) from astronomy to Raman imaging, it becomes possible to record entire Raman images quickly within a single exposure, without the need for a tedious scanning procedure. An IFS-based Raman imaging setup is presented, which is capable of measuring skin ex vivo or in vivo. It is demonstrated how Raman images of healthy and cancerous skin biopsies were recorded and analyzed.
After having demonstrated that an IFU, attached to a microscope rather than to a telescope, is capable of differentiating complex organic tissue with spatially resolved Raman spectroscopy, we have launched a clinical validation program that utilizes a novel optimized fiber-coupled multi-channel spectrograph whose layout is based on the modular MUSE spectrograph concept. The new design features a telecentric input and has an extended blue performance, but otherwise maintains the properties of high throughput and excellent image quality over an octave of wavelength coverage with modest spectral resolution. We present the opto-mechanical layout and details of its optical performance.
We here report on recent progress on astronomical optical frequency comb generation at innoFSPEC-Potsdam and
present preliminary test results using the fiber-fed Multi Unit Spectroscopic Explorer (MUSE) spectrograph. The
frequency comb is generated by propagating two free-running lasers at 1554.3 and 1558.9 nm through two dispersionoptimized
nonlinear fibers. The generated comb is centered at 1590 nm and comprises more than one hundred lines with
an optical-signal-to-noise ratio larger than 30 dB. A nonlinear crystal is used to frequency double the whole comb
spectrum, which is efficiently converted into the 800 nm spectral band. We evaluate first the wavelength stability using
an optical spectrum analyzer with 0.02 nm resolution and wavelength grid of 0.01 nm. After confirming the stability
within 0.01 nm, we compare the spectra of the astro-comb and the Ne and Hg calibration lamps: the astro-comb exhibits
a much larger number of lines than lamp calibration sources. A series of preliminary tests using a fiber-fed MUSE
spectrograph are subsequently carried out with the main goal of assessing the equidistancy of the comb lines. Using a
P3d data reduction software we determine the centroid and the width of each comb line (for each of the 400 fibers
feeding the spectrograph): equidistancy is confirmed with an absolute accuracy of 0.4 pm.