Dermal filler injections are common skin cosmetic procedure, but intravascular injection of fillers can lead to various complications. Therefore, real-time imaging of vessels and fillers will be helpful to lower the complications. In this study, angiographic optical coherence tomography (A-OCT) was used for real-time visualization of vessels and fillers. This method was demonstrated in an in vivo mouse model by monitoring filler injection in the tail. The locations of main artery and fillers were identified by A-OCT imaging before and after injection. Our study suggests that the real-time angiographic OCT can serve as a useful tool for filler injection guide.