The relationship between the transmission distance and the number of Fresnel zone plate rings is evaluated. The influence of different transmission distance and the number of Fresnel zone-plate rings are analyzed. Their parameters of the best reconstructed image are obtained. A point light source is taken as an example, a phase-only spatial light modulator with the resolution of 3840×2160 pixels and the pixel interval of 3.74 μm is used for experimental verification. The numerical simulation and optical experiment results show that the optimal reconstructed image has a transmission distance of 200 mm. Meanwhile, the optimal Fresnel zone ring number is 8 under the same conditions. This study provides the optimal parameters for the spatial light modulators with different size.
Digital holography can reconstruct 3-D data cube from a 2-D hologram for the tomographic imaging. Digital one-shot inline holography (DOIH) maintains the maximal space-bandwidth product compared with off-axis holography and keeps both amplitude and phase in the interference pattern. DOIH often suffers from intrinsic defects such as twin-image interruption and squared noise. In this work, compressive sensing is applied in the tomographic reconstruction to overcome the defects. The designed algorithm based on compressive DOIH demonstrates the feasibility in removing the squared noise from a single 2-D in-line hologram.
Biochips have been an advanced technology for biomedical applications since the end of the 20th century. Optical detection systems have been a very important tool in biochip analysis. Microscopes are often inadequate for high resolution and big view-area detection of microarray chips, thus some new optical instruments are required. In this work, a novel digital imaging scanning system with dark-field irradiation is developed for some biomedical applications for microarray chips, characterized by analyzing genes and proteins of clinical samples with high specific, parallel, and nanoliter samples. The novel optical system has a high numerical aperture (NA=0.72), a long working distance (wd>3.0 mm), an excellent contrast and signal-to-noise ratio, a high resolving power close to 3 µm, and an efficiency of collected fluorescence more than two-fold better than that of other commercial confocal biochip scanners. An edge overlap algorithm is proposed for the image restructure of free area detection and correcting scanning position errors to a precision of 1 pixel. A novel algorithm is explored for recognizing the target from the scanning images conveniently, removing noise, and producing the signal matrix of biochip analysis. The digital imaging scanning system is equally as good for the detection of enclosed biochips as it is for the detection of biological samples on a slide surface covered with a glass cover slip or in culture solution. The clinical bacteria identification and serum antibody detection of biochips are described.