Mr. Chenfeng Ji Profile

Chenfeng Ji

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Proceedings Article | 6 March 2009 Paper

Analysis of relationship between apoptosis and change of Ca2+ in HepG-2 induced by CSA with laser scanning confocal technology

Yu-bin Ji, Lei Yu

Proceedings Volume 7280, 72800T (2009) https://doi.org/10.1117/12.821311

KEYWORDS: Cell death, Laser scanners, Microscopes, Luminescence, Confocal microscopy, Tumors, Laser applications, Calcium, Analytical research, Carbon dioxide

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Laser scanning confoncal technology was used to study relationship between apoptosis and change of Ca²⁺ induced by CSA (Capparis spinosa L. total alkaloid, CSA) on human heptocarcinoma cell HepG-2. Killing effect of CSA on human heptocarcinoma cell HepG-2 was measured by MTT method, while morphological observation of HepG-2 cells was completed by fluorescence microscope. Apoptosis induced by CSA on HepG-2 cells was measured by flowcytometry. In addition, change of intracellular Ca²⁺ level of CSA on HepG-2 cells was observed by laser scanning confocal microscope. As a result, CSA had obvious cytotoxicity on HepG-2 in a dose-dependent manner, and its IC50 was 162.4μg/ml. CSA could induce characteristic apoptosic morphology of HepG-2 cells, and apoptosis percentage was significantly higher than control one. Migration of cells cycle from S phase to G2 phase had been blocked by CSA. Concentration of Ca²⁺ in HepG-2 had been increased by CSA, which was positive correlation with drug dosage. CSA had obvious effect of killing and inducing apoptosis on human heptocarcinoma cell HepG-2, and overload of Ca²⁺ might be invovled in these events.

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