Laser scanning confoncal technology was used to study relationship between apoptosis and change of Ca<sup>2+</sup> induced by
CSA (Capparis spinosa L. total alkaloid, CSA) on human heptocarcinoma cell HepG-2. Killing effect of CSA on
human heptocarcinoma cell HepG-2 was measured by MTT method, while morphological observation of HepG-2 cells
was completed by fluorescence microscope. Apoptosis induced by CSA on HepG-2 cells was measured by
flowcytometry. In addition, change of intracellular Ca<sup>2+</sup> level of CSA on HepG-2 cells was observed by laser scanning
confocal microscope. As a result, CSA had obvious cytotoxicity on HepG-2 in a dose-dependent manner, and its IC50
was 162.4μg/ml. CSA could induce characteristic apoptosic morphology of HepG-2 cells, and apoptosis percentage was
significantly higher than control one. Migration of cells cycle from S phase to G2 phase had been blocked by CSA.
Concentration of Ca<sup>2+</sup> in HepG-2 had been increased by CSA, which was positive correlation with drug dosage. CSA
had obvious effect of killing and inducing apoptosis on human heptocarcinoma cell HepG-2, and overload of Ca<sup>2+</sup> might
be invovled in these events.