We propose a high-throughput 3D imaging cytometer for fast quantification of DNA double strand break (DSB) frequency in cells for DNA damage study. With structured illumination enabled depth contrast and a fast focus tunable lens enabled scanning, this system generates a three-dimensional stack of clustered nuclei γH2AX foci with submicron resolution at a speed of 800 cells/second. Moreover, we unify the stack construction with the deep neural network, which largely improve quantification accuracy as well as the processing speed. Compared to previous 2D imaging approach, the addition of z-resolution in our 3D method provides an extra dimension of contrast and thus allows for more accurate DNA DSB quantification.
Diffraction Phase Microscopy (DPM) is a widely used quantitative phase imaging method, whose common-path nature endows it with low noise and high sensitivity. Current applications of DPM include biological topography as well as biological dynamics for its nondestructive feature. Many different forms of DPM based on the original idea have appeared according to the different demands. In this paper, both the principle and the DPM classification will be given a comprehensive description. Furthermore, the future trend of DPM development is also discussed.