Raman spectroscopy and Multivariate methods were used to study serum blood samples of control and breast cancer
patients. Blood samples were obtained from 11 patients and 12 controls from the central region of Mexico. Our results
show that principal component analysis is able to discriminate serum sample of breast cancer patients from those of
control group, also the loading vectors of PCA plotted as a function of Raman shift shown which bands permitted to
make the maximum discrimination between both groups of samples.
Clinical diagnosis of infections, generally are realized by serological methods, which identifies the antibodies presents
in serum or tissue fluids of the patient. Antibodies are proteins present in our bodies that aid in the elimination of
pathogens or antigens. Identification of antibodies isotypes is important because can help to predict when and whether
patients will recover from infections and are commonly diagnosed by means of indirect methods such as serological test. In the other hand, the majority of these methods requires specific kits for the analysis, special sample preparation, chemical reagents, expensive equipment and require long time for getting results. In this work we show the feasibility to discriminate antibody isotypes in biological fluids like human colostrum by means of Raman spectroscopy and chemometrics. Spectra were obtained using an excitation wavelength of 514 nm over dried samples of human colostrum labeled previously as positives to specific IgG and IgM antibodies against Toxoplasma Gondii by means of ELISA test. Partial least square-discriminant analysis (PLS-DA) was used to discriminate among antibody isotypes by use second derivative of Raman spectra of colostrum samples.
More than 60 million people in the United States and 23 million people in Mexico probably are infected with the Toxoplasma parasite, but very few have symptoms because the immune system usually keeps the parasite from causing illness. However, for people whose immune system is compromised, the consequences can be fatal. Toxoplasmosis is detected indirectly by different serological tests, where the sample requires a previous preparation. We analyze the feasibility to use Raman spectroscopy and principal component analysis (PCA) as an alternative method to detect the presence or absence of antibodies IgG (immunoglobulin G), IgM (immunoglobulin M), and IgA (immunoglobulin A), against Toxoplasma gondii, in a simple and fast way, in samples of human colostrum from a group of volunteers who were in contact with the parasite and others who were not in contact with the parasite.
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