Mr. Dan Butmalai Profile

Dan Butmalai

SPIE Involvement:

Author

Publications (1)

This will count as one of your downloads.

You will have access to both the presentation and article (if available).

DOWNLOAD NOW

This content is available for download via your institution's subscription. To access this item, please sign in to your personal account.

Email or Username Forgot your username?

Password Forgot your password?

Show

Keep me signed in

No SPIE account? Create an account

Proceedings Article | 22 July 2019 Paper

Optical biomarkers for detection of malignant tissue using digital holographic microscopy

Violeta Calin, Mona Mihailescu, Radu Costea, Adrian Dumitru, Oana-Maria Patrascu, Felix Brehar, George Petrescu, Mihai Lisievici, Brindus Comanescu, Viorela Grigore, Dan Butmalai, Tudor Savopol, Mihaela Moisescu

Proc. SPIE. 11076, Advances in Microscopic Imaging II

KEYWORDS: Tissues, Digital holography, Holography, Tissue optics, Biopsy, Microscopy, Phase shifts, Tumors, Statistical analysis

Read Abstract +

The histopathological diagnosis in malignances requests well trained specialists and multi-step operational procedures for sample preparation. Faster and more objective evaluation protocols should be implemented to give support to the pathologists. The Quantitative Phase Imaging based methods are biological-proved to be efficient in revealing important characteristics of the living structures without any labeling. These can be further exploited for an automatic evaluation of complex tissues. Using an off-axis Digital Holographic Microscopy setup, biopsies of two histological origins: cerebral (grade II glioma and grade IV glioblastoma) and colonic malignancies (dysplastic and malignant colonic adenomatous polyps), were investigated. Various parameters of quantitative phase shift maps (QPMs) were computed (mean, variance, median, kurtosis, skewness, energy, entropy). The possibility of automatic discrimination of tumor tissues having different structural complexity and presenting various malignancy grades was evaluated using supervised machine learning algorithms. The analysis of phase shift maps has successfully discriminated between levels of malignancy with high statistical confidence in the case of gliomas. Moreover an algorithm with the ability to classify the tissue biopsies in different malignant stages using parameters based on QPMs has been implemented on glioma tissues having a high level of homogeneity. In case of colonic polyps, the heterogeneity of the multilayered tissue demanded QPMs analysis to be performed on selected area of interest even though some statistical differences were obtained for global evaluation of phase shift distributions. In case of colonic polyps, for a good accuracy of classification algorithm a larger library of QPMs is under construction.

My Library

You currently do not have any folders to save your paper to! Create a new folder below.

Folder Name

Folder Description

View contact details

UPDATE YOUR PROFILE

Is this your profile? Update it now.

Sign into your SPIE.org account

Don’t have a profile and want one?

Create an account on SPIE.org

Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks. You are receiving this notice because your organization may not have SPIE eBooks access.*

*Shibboleth/Open Athens users─please sign in to access your institution's subscriptions.

To obtain this item, you may purchase the complete book in print format on SPIE.org.

PERSONAL SIGN IN
Full access may be available with your subscription

Email or Username Forgot your username?

Password Forgot your password?

Show

Keep me signed in

No SPIE account? Create an account

Institutional Access:

PURCHASE THIS CONTENT

INTERESTED IN A FREE CORPORATE TRIAL?

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members:

Non-members: ADD TO CART

Keywords/Phrases

Search In:

Publication Years