Dr. Daniel L. Farkas Profile

Dr. Daniel L. Farkas

Chairman at SafetySpect Inc

SPIE Involvement:

Track Chair | Editor | Author

Publications (59)

Proceedings Article | 14 May 2019 Presentation

Multimode optical imaging for identification of fish fillet substitution and mislabeling (Conference Presentation)

Fartash Vasefi, Rachel Isaacs , Stas Sokolov, Li Kang, Rosalee Hellberg , Daniel Farkas , Jianwei Qin , Diane Chan , Moon Kim

Proc. SPIE. 11016, Sensing for Agriculture and Food Quality and Safety XI

KEYWORDS: Optical imaging, Near infrared, Short wave infrared radiation, Visible radiation, Imaging systems, Image processing, Luminescence, Reflectivity, Imaging spectroscopy, Raman spectroscopy

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Proceedings Article | 4 March 2019 Presentation

Ultra-resolution spectral confocal add-on systems for light microscopes (Conference Presentation)

Fartash Vasefi, Kevin Burton, Nicholas Booth, Daniel Farkas

Proc. SPIE. 10881, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVII

KEYWORDS: Confocal microscopy, Microscopes, Pathology, Laser scanners, Spectral resolution, Image enhancement, Molecular biology, Fluorescence resonance energy transfer, Laser tissue interaction, Resonance enhancement

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Proceedings Article | 11 June 2018 Presentation

Mie scattering characterization by ultra-spectral illumination using micro-sized tunable liquid crystal optical filters (Conference Presentation)

Fartash Vasefi, Russell Barbour, Caleb Stoltzfus, Zeb Barber, Daniel Farkas

Proc. SPIE. 10497, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVI

KEYWORDS: Confocal microscopy, Optical filters, Tissues, Scattering, Particles, Light scattering, Liquid crystals, Mie scattering, Biological research, Optical microcavities

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Proceedings Article | 15 May 2018 Presentation

Multimode optical imaging for food quality and safety applications (Conference Presentation)

Fartash Vasefi, Danielle Rosen, Nicholas Booth, Hesam Hafizi, Li Kang, Rosalee Hellberg, Daniel Farkas

Proc. SPIE. 10665, Sensing for Agriculture and Food Quality and Safety X

KEYWORDS: Optical imaging, Hyperspectral imaging, Light sources, Light emitting diodes, LED lighting, Safety, Imaging systems, Luminescence, Reflectivity, Image acquisition

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Proceedings Article | 24 April 2017 Presentation

In vivo features of melanocytic lesions: multimode hyperspectral dermoscopy, reflectance confocal microscopy, and histopathologic correlates (Conference Presentation)

Fartash Vasefi, Nicholas MacKinnon, Manu Jain, Miguel Cordova, Kivanc Kose, Milind Rajadhyaksha, Allan Halpern, Daniel Farkas

Proc. SPIE. 10068, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XV

KEYWORDS: Confocal microscopy, Cancer, Imaging systems, Skin, Reflectivity, Diagnostics, Melanoma, In vivo imaging, Standards development, 3D image processing

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Proceedings Article | 24 April 2017 Presentation

Improved heuristics for early melanoma detection using multimode hyperspectral dermoscopy (Conference Presentation)

Fartash Vasefi, Nicholas MacKinnon, Nicholas Booth, Daniel Farkas

Proc. SPIE. 10068, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XV

KEYWORDS: Hyperspectral imaging, Polarization, Imaging systems, Skin, Luminescence, Feature extraction, Collagen, Melanoma, In vivo imaging, Absorption

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SPIE Journal Paper | 26 December 2016

Review of the potential of optical technologies for cancer diagnosis in neurosurgery: a step toward intraoperative neurophotonics

Fartash Vasefi, Nicholas MacKinnon, Daniel Farkas, Babak Kateb

NPh Vol. 4 Issue 01

KEYWORDS: Tumors, Tissues, Brain, Tissue optics, Luminescence, Raman spectroscopy, Cancer, Neurophotonics, Neuroimaging, Optical coherence tomography

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SPIE Journal Paper | 10 November 2016

Separating melanin from hemodynamics in nevi using multimode hyperspectral dermoscopy and spatial frequency domain spectroscopy

Fartash Vasefi, Nicholas MacKinnon, Rolf Saager, Kristen Kelly, Tyler Maly, Nicholas Booth, Anthony Durkin, Daniel Farkas

JBO Vol. 21 Issue 11

KEYWORDS: Skin, Absorption, Hemodynamics, Tissue optics, Ultrasonography, Spectroscopy, Melanoma, Signal attenuation, Polarization, Imaging systems

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Proceedings Article | 6 April 2016 Paper

Multimode optical dermoscopy (SkinSpect) analysis for skin with melanocytic nevus

Fartash Vasefi, Nicholas MacKinnon, Rolf Saager, Kristen Kelly, Tyler Maly, Robert Chave, Nicholas Booth, Anthony Durkin, Daniel Farkas

Proc. SPIE. 9711, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX

KEYWORDS: Hyperspectral imaging, Statistical analysis, Modulation, Tissues, Skin, Melanoma, Biological research, In vivo imaging, Tissue optics, Spectroscopes

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Proceedings Article | 6 April 2016 Presentation + Paper

Melanoma detection using smartphone and multimode hyperspectral imaging

Nicholas MacKinnon, Fartash Vasefi, Nicholas Booth, Daniel Farkas

Proc. SPIE. 9711, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX

KEYWORDS: Hyperspectral imaging, Medicine, Polarization, Tissues, Imaging systems, Dermatology, Skin, Melanoma, Image quality, RGB color model

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Proceedings Article | 25 March 2015 Paper

Quantifying the optical properties of turbid media using polarization sensitive hyperspectral imaging (SkinSpect): two-layer optical phantom studies

Fartash Vasefi, Nicholas MacKinnon, Rolf Saager, Anthony Durkin, Robert Chave, Daniel Farkas

Proc. SPIE. 9328, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIII

KEYWORDS: Hyperspectral imaging, Polarization, Optical properties, Imaging systems, Scattering, Signal attenuation, Skin, Melanoma, Tissue optics, Absorption

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SPIE Journal Paper | 21 March 2014

High-speed multispectral confocal biomedical imaging

Gary Carver, Sarah Locknar, William Morrison, V. Krishnan Ramanujan, Daniel Farkas

JBO Vol. 19 Issue 03

KEYWORDS: Confocal microscopy, Tissues, Multispectral imaging, Biomedical optics, Luminescence, Spectroscopy, Tumors, Endoscopy, Sensors, Microscopy

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Proceedings Article | 4 March 2014 Paper

Toward in vivo diagnosis of skin cancer using multimode imaging dermoscopy: (II) molecular mapping of highly pigmented lesions

Fartash Vasefi, Nicholas MacKinnon, Daniel Farkas

Proc. SPIE. 8947, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII

KEYWORDS: Hyperspectral imaging, Polarization, Signal attenuation, Blood, Skin, Chromophores, Absorbance, Tissue optics, RGB color model, Absorption

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Proceedings Article | 4 March 2014 Paper

Toward in vivo diagnosis of skin cancer using multimode imaging dermoscopy: (I) clinical system development and validation

Nicholas MacKinnon, Fartash Vasefi, Daniel Farkas

Proc. SPIE. 8947, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII

KEYWORDS: Hyperspectral imaging, Polarization, Tissues, Imaging systems, Cameras, Skin, Luminescence, Skin cancer, Melanoma, Prototyping

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Proceedings Article | 4 March 2014 Paper

Multispectral imaging for diagnosis and treatment

Gary Carver, Sarah Locknar, William Morrison, Daniel Farkas

Proc. SPIE. 8947, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII

KEYWORDS: Confocal microscopy, Endoscopy, Tissues, Spectroscopy, Luminescence, Multispectral imaging, Software development, Spatial resolution, Tissue optics, Laser tissue interaction

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Proceedings Article | 26 February 2013 Paper

Quantifying the optical properties and chromophore concentrations of turbid media using polarization sensitive hyperspectral imaging: optical phantom studies

Fartash Vasefi, Rolf Saager, Anthony Durkin, Nicholas MacKinnon, Eugene Gussakovsky, Robert Chave, Daniel Farkas

Proc. SPIE. 8587, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XI

KEYWORDS: Hyperspectral imaging, Near infrared, Titanium dioxide, Polarization, Optical properties, Imaging systems, Scattering, Skin, Tissue optics, Absorption

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Proceedings Article | 22 February 2013 Paper

In vivo skin chromophore mapping using a multimode imaging dermoscope (SkinSpect™)

Nicholas MacKinnon, Fartash Vasefi, Eugene Gussakovsky, Gregory Bearman, Robert Chave, Daniel Farkas

Proc. SPIE. 8587, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XI

KEYWORDS: Data modeling, Polarization, Tissues, Skin, Luminescence, Reflectivity, Chromophores, Melanoma, Tissue optics, RGB color model

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Proceedings Article | 22 February 2013 Paper

High-speed multispectral confocal imaging

Gary Carver, Sarah Locknar, William Morrison, Daniel Farkas

Proc. SPIE. 8587, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XI

KEYWORDS: Confocal microscopy, Endoscopy, Tissues, Sensors, Spectroscopy, Microscopy, Luminescence, Multispectral imaging, Spatial resolution, Chemical elements

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SPIE Journal Paper | 6 February 2012

Investigating photoexcitation-induced mitochondrial damage by chemotherapeutic corroles using multimode optical imaging

Jae-Youn Hwang, Daniel Farkas, David Lubow, Jessica Sims, Lali Medina-Kauwe, Harry Gray, Atif Mahammed, Zeev Gross

JBO Vol. 17 Issue 1

KEYWORDS: Luminescence, Tumors, Digital image correlation, Optical imaging, Picosecond phenomena, Fluorescence lifetime imaging, Toxicity, Cancer, Imaging spectroscopy, Absorption

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SPIE Journal Paper | 1 June 2011

Ratiometric spectral imaging for fast tumor detection and chemotherapy monitoring in vivo

Jae-Youn Hwang, Daniel Farkas, Lali Medina-Kauwe, Zeev Gross, Harry Gray

JBO Vol. 16 Issue 06

KEYWORDS: Tumors, Imaging spectroscopy, Luminescence, In vivo imaging, Image classification, Molecules, Breast, Tissues, Skin, Gallium

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Proceedings Article | 28 February 2011 Paper

Polarization-sensitive digital dermoscopy for image processing-assisted evaluation of atypical nevi: towards step-wise detection of melanoma

Lauren Yu, Anika O. N. Joseph, Erik Lindsley, Daniel Farkas

Proc. SPIE. 7902, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX

KEYWORDS: Hyperspectral imaging, Digital image processing, Detection and tracking algorithms, Image processing, Skin, Image analysis, Melanoma, Medical imaging, Fractal analysis, Algorithm development

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Proceedings Article | 28 February 2011 Paper

Multimode optical imaging for translational chemotherapy: in vivo tumor detection and delineation by targeted gallium corroles

Jae Youn Hwang, Zeev Gross, Harry Gray, Lali Medina-Kauwe, Daniel Farkas

Proc. SPIE. 7902, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX

KEYWORDS: Target detection, Optical imaging, Tumors, Tissues, Imaging systems, Luminescence, Imaging spectroscopy, Fluorescence lifetime imaging, In vivo imaging, Gallium

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Proceedings Article | 18 February 2011 Paper

Investigating the photosensitizer-potential of targeted gallium corrole using multimode optical imaging

Jae Youn Hwang, Jay Lubow, David Chu, Zeev Gross, Harry Gray, Daniel Farkas, Lali Medina-Kauwe

Proc. SPIE. 7886, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XX

KEYWORDS: Optical imaging, Confocal microscopy, Proteins, Photodynamic therapy, Breast cancer, Tumors, Luminescence, Imaging spectroscopy, Fluorescence lifetime imaging, Photosensitizer targeting

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SPIE Journal Paper | 1 September 2010

Biomedical Optical Imaging

Barry Masters, James Fujimoto, Daniel Farkas

JBO Vol. 15 Issue 05

KEYWORDS: Biomedical optics, Optical imaging, Medical research, Optical coherence tomography, Confocal microscopy, Ophthalmology, Tissue optics, Optical microscopy, Imaging spectroscopy, Microscopy

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Proceedings Article | 9 March 2010 Paper

Automatic recognition of abnormal cells in cytological tests using multispectral imaging

A. Gertych, G. Galliano, S. Bose, D. Farkas

Proc. SPIE. 7624, Medical Imaging 2010: Computer-Aided Diagnosis

KEYWORDS: Imaging systems, Image segmentation, Image processing, Pathology, Feature extraction, Multispectral imaging, Image classification, Cervical cancer, Binary data, Library classification systems

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Proceedings Article | 25 February 2010 Paper

Real-time megapixel multispectral bioimaging

Jason Eichenholz, Nick Barnett, Yishung Juang, Dave Fish, Steve Spano, Erik Lindsley, Daniel Farkas

Proc. SPIE. 7568, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII

KEYWORDS: Optical filters, Imaging systems, Cameras, Image processing, Digital filtering, Imaging spectroscopy, Multispectral imaging, Image filtering, Charge-coupled devices, Radio frequency weapons

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SPIE Journal Paper | 1 November 2008

Quantitative and morphometric evaluation of the angiogenic effects of leptin

Dodanim Talavera-Adame, Yizhi Xiong, Tong Zhao, Ana Arias, M. Rocio Sierra-Honigmann, Daniel Farkas

JBO Vol. 13 Issue 06

KEYWORDS: Content addressable memory, Image analysis, Control systems, Blood vessels, Molecules, In vivo imaging, Surgery, MATLAB, Fractal analysis, Image processing

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Proceedings Article | 18 March 2008 Paper

Large field of view scanning fluorescence lifetime imaging system for multimode optical imaging of small animals

Jae Youn Hwang, Hasmik Agadjanian, Lali Medina-Kauwe, Zeev Gross, Harry Gray, Karn Sorasaenee, Daniel Farkas

Proc. SPIE. 6859, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VI

KEYWORDS: Optical imaging, Tissues, Imaging systems, Luminescence, Molecules, Imaging spectroscopy, Fluorescence lifetime imaging, Optical scanning systems, Pulsed laser operation, Preclinical imaging

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Proceedings Article | 28 February 2008 Paper

Characterization of tumor cells and stem cells by differential nuclear methylation imaging

Jian Tajbakhsh, Kolja Wawrowsky, Arkadiusz Gertych, Ori Bar-Nur, Eugene Vishnevsky, Erik Lindsley, Daniel Farkas

Proc. SPIE. 6859, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VI

KEYWORDS: Confocal microscopy, Cancer, Tumors, Visualization, Surgery, Digital filtering, Image analysis, Image filtering, Stem cells, 3D image processing

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Proceedings Article | 25 February 2008 Paper

Intelligent noncontact surgeon-computer interface using hand gesture recognition

Michael Zhao, Andreas Nowatzyk, Thomas Lu, Daniel Farkas

Proc. SPIE. 6805, Three-Dimensional Image Capture and Applications 2008

KEYWORDS: Human-machine interfaces, Surgery, Cameras, Feature extraction, Light sources and illumination, Neural networks, Algorithm development, Gesture recognition, 3D image processing, Evolutionary algorithms

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Proceedings Article | 21 February 2008 Paper

Quantitative endoscopic imaging elastic scattering spectroscopy: model system/tissue phantom validation

E. Lindsley, D. Farkas

Proc. SPIE. 6870, Design and Performance Validation of Phantoms Used in Conjunction with Optical Measurements of Tissue

KEYWORDS: Endoscopy, Optical spheres, Cancer, Data modeling, Titanium dioxide, Tissues, Scattering, Imaging spectroscopy, Mie scattering, Tissue optics

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Proceedings Article | 19 February 2007 Paper

Contrast enhancement in biomedical optical imaging using ultrabright color LEDs

Kevin Burton, Raphael Zelikowsky, Danny Shandling, Erik Lindsley, Daniel Farkas

Proc. SPIE. 6441, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V

KEYWORDS: Endoscopy, Microscopes, Light emitting diodes, LED lighting, Tissues, Spatial frequencies, Microscopy, Tungsten, Lamps, Endoscopes

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Proceedings Article | 19 February 2007 Paper

Multimode optical imaging of small animals: development and applications

J. Y. Hwang, C. Moffatt-Blue, O. Equils, M. Fujita, J. Jeong, N. Khazenzon, E. Lindsley, J. Ljubimova, A. Nowatzyk, D. Farkas, S. Wachsmann-Hogiu

Proc. SPIE. 6441, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V

KEYWORDS: Optical imaging, Breast, Hyperspectral imaging, Tumors, Tissues, Imaging systems, Luminescence, Charge-coupled devices, Fluorescence lifetime imaging, Bioluminescence

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Proceedings Article | 19 February 2007 Paper

Wide-field two-photon microscopy: features and advantages for biomedical applications

S. Wachsmann-Hogiu, J. Hwang, E. Lindsley, D. Farkas

Proc. SPIE. 6441, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V

KEYWORDS: Confocal microscopy, Biomedical optics, Tissues, Blood, Microscopy, Luminescence, Objectives, Fluorescence lifetime imaging, Picosecond phenomena, Two photon excitation microscopy

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Proceedings Article | 19 February 2007 Paper

Intelligent spectral signature bio-imaging in vivo for surgical applications

Jihoon Jeong, Philip Frykman, Mark Gaon, Alice Chung, Erik Lindsley, Jae Y. Hwang, Daniel Farkas

Proc. SPIE. 6441, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V

KEYWORDS: Neck, Visualization, Tissues, Surgery, Databases, Image processing, Imaging spectroscopy, Colon, In vivo imaging, Animal model studies

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Proceedings Article | 22 February 2006 Paper

Spectral imaging detects breast cancer in fresh unstained specimens

Alice Chung, Mark Gaon, Jihoon Jeong, Scott Karlan, Erik Lindsley, Sebastian Wachsmann-Hogiu, Yizhi Xiong, Tong Zhao, Daniel Farkas

Proc. SPIE. 6088, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV

KEYWORDS: Breast, Cancer, Breast cancer, Tumors, Tissues, Image segmentation, Imaging spectroscopy, Image classification, In vivo imaging, Library classification systems

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Proceedings Article | 21 February 2006 Paper

Acousto-optic tuneable filters: advances and applications to microscopy

C. Pannell, E. Wachman, D. Farkas, J. Ward, W. Seale

Proc. SPIE. 6088, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV

KEYWORDS: Diffraction, Optical filters, Scattering, Crystals, Image quality, Transducers, Image filtering, Tunable filters, Acoustics, Acousto-optics

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Proceedings Article | 29 April 2005 Paper

A novel node-structural map for angiogenesis analysis

Yizhi Xiong, Tong Zhao, Dodanim Talavera, Rocio Honigmann, Daniel Farkas

Proc. SPIE. 5747, Medical Imaging 2005: Image Processing

KEYWORDS: Blood vessels, Image segmentation, Control systems, Arteries, Image analysis, Quantitative analysis, Fractal analysis, In vivo imaging, Content addressable memory, In vitro testing

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Proceedings Article | 29 March 2005 Paper

Multiphoton, confocal, and lifetime microscopy for molecular imaging in cartilage

Sebastian Wachsmann-Hogiu, Deborah Krakow, Veneta Kirilova, Daniel Cohn, Cristina Bertolotto, Dora Acuna, Qiyin Fang, Nikola Krivorov, Daniel Farkas

Proc. SPIE. 5699, Imaging, Manipulation, and Analysis of Biomolecules and Cells: Fundamentals and Applications III

KEYWORDS: Confocal microscopy, Proteins, Multiphoton microscopy, Cartilage, Tissues, Microscopy, Luminescence, Fluorescence lifetime imaging, Molecular imaging, Fluorescence resonance energy transfer

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Proceedings Article | 29 March 2005 Paper

Improved 3D cellular imaging by multispectral focus assessment

Tong Zhao, Yizhi Xiong, Alice Chung, Elliot Wachman, Daniel Farkas

Proc. SPIE. 5699, Imaging, Manipulation, and Analysis of Biomolecules and Cells: Fundamentals and Applications III

KEYWORDS: Microscopes, Point spread functions, Databases, Stereoscopy, Image segmentation, Image processing, Image analysis, Multispectral imaging, Solids, 3D image processing

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Proceedings Article | 1 July 2004 Paper

Confocal and two-photon imaging in cartilage: expression patterns of Filamin A and B

Sebastian Wachsmann-Hogiu, Deborah Krakow, Eiman Sebald, Cristina Bertolotto, Dora Acuna, Daniel Farkas

Proc. SPIE. 5322, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues II

KEYWORDS: Confocal microscopy, Two photon imaging, Proteins, Cartilage, Tissues, Microscopy, Luminescence, Laser scanners, Objectives, Digital image correlation

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Proceedings Article | 1 July 2004 Paper

A recursive spectral selection scheme for unsupervised segmentation of multispectral Pap smear image sets

Tong Zhao, Elliot Wachman, Stanley Geyer, Daniel Farkas

Proc. SPIE. 5322, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues II

KEYWORDS: Principal component analysis, Computer aided diagnosis and therapy, Data modeling, Tissues, Image segmentation, Multispectral imaging, Distance measurement, Machine learning, Feature selection, Cervical cancer

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Proceedings Article | 1 July 2004 Paper

A novel scheme for abnormal cell detection in Pap smear images

Tong Zhao, Elliot Wachman, Daniel Farkas

Proc. SPIE. 5318, Advanced Biomedical and Clinical Diagnostic Systems II

KEYWORDS: Statistical analysis, Cancer, Imaging systems, Wavelets, Feature extraction, Multispectral imaging, Distance measurement, Neural networks, Image classification, Cervical cancer

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Proceedings Article | 1 July 2004 Paper

The hyperspectral imaging endoscope: a new tool for in vivo cancer detection

Erik Lindsley, Elliot Wachman, Daniel Farkas

Proc. SPIE. 5322, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues II

KEYWORDS: Cancer, Tissues, Imaging systems, Scattering, Cameras, Endoscopes, Biopsy, Mie scattering, In vivo imaging, Tissue optics

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Proceedings Article | 28 May 2002 Paper

Thermal energy effects on articular cartilage: a multidisciplinary evaluation

Lee Kaplan, John Ernsthausen, Dan Ionescu, Rebecca Studer, James Bradley, Constance Chu, Freddie Fu, Daniel Farkas

Proc. SPIE. 4622, Optical Diagnostics of Living Cells V

KEYWORDS: Optical imaging, Microscopes, Cartilage, Tissues, Surgery, Manufacturing, Control systems, Thermal effects, Thermal modeling, Temperature metrology

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SPIE Journal Paper | 1 January 2001

Rhod-2 based measurements of intracellular calcium in the perfused mouse heart: cellular and subcellular localization and response to positive intropy

Guy MacGowan, Congwu Du, Vazha Glonty, Joseph Suhan, Daniel Farkas, Alan Koretsky

JBO Vol. 6 Issue 01

KEYWORDS: Calcium, Luminescence, Heart, Absorbance, Confocal microscopy, Microscopy, Tissues, Photomicroscopy, Electron microscopy, Motion measurement

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Proceedings Article | 11 October 2000 Paper

Noninvasive imaging of biological tissue structure, function, and abnormalities with optical coherence tomography

Yingtian Pan, John Lavelle, S. Bastaky, Daniel Farkas, Mark Zeidel

Proc. SPIE. 4224, Biomedical Photonics and Optoelectronic Imaging

KEYWORDS: Mirrors, Tumors, Tissues, Imaging systems, Scattering, Optical coherence tomography, Image resolution, Biopsy, Bladder, Tissue optics

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Proceedings Article | 3 May 1999 Paper

Quantitative measurement of calcium transients in perfused mouse heart using Rhod-2 fluorometry

Congwu Du, Guy MacGowan, Daniel Farkas, Alan Koretsky

Proc. SPIE. 3602, Advances in Fluorescence Sensing Technology IV

KEYWORDS: Tissues, Scattering, Calibration, Luminescence, Calcium, Heart, Absorbance, Tissue optics, Signal detection, Absorption

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Proceedings Article | 16 October 1998 Paper

Spectral imaging in biomedicine: a selective overview

Richard Levenson, Elliot Wachman, Wenhua Niu, Daniel Farkas

Proc. SPIE. 3438, Imaging Spectrometry IV

KEYWORDS: Infrared imaging, Optical filters, Tissues, Pathology, Spectroscopy, Luminescence, Imaging spectroscopy, Image filtering, Infrared radiation, Tunable filters

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SPIE Journal Paper | 1 October 1998

Non-invasive Imaging of Living Human Skin with Dual-wavelength Optical Coherence Tomography in Two and Three Dimensions

Yingtian Pan, Daniel Farkas

JBO Vol. 3 Issue 04

KEYWORDS: Optical coherence tomography, Tissues, Skin, 3D image processing, Scattering, Light scattering, In vivo imaging, Blood vessels, Image resolution, Scanners

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Proceedings Article | 29 April 1998 Paper

Noninvasive imaging of biological tissue with dual-wavelength optical coherence tomography

Yingtian Pan, Daniel Farkas

Proc. SPIE. 3260, Optical Investigations of Cells In Vitro and In Vivo

KEYWORDS: Light sources, Biomedical optics, Fiber optics, Tissues, Speckle, Scattering, Optical coherence tomography, Scanners, Tomography, Tissue optics

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Proceedings Article | 23 May 1997 Paper

In-vivo imaging of biological tissues using 1.3 um optical coherence tomography

Yingtian Pan, Daniel Farkas

Proc. SPIE. 2983, Functional Imaging and Optical Manipulation of Living Cells

KEYWORDS: Coherence imaging, Mirrors, Tissues, Scattering, Optical coherence tomography, Skin, Diagnostics, Image resolution, In vivo imaging, Tissue optics

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Proceedings Article | 23 May 1997 Paper

Digital spectral imaging for histopathology and cytopathology

Richard Levenson, Daniel Farkas

Proc. SPIE. 2983, Functional Imaging and Optical Manipulation of Living Cells

KEYWORDS: Statistical analysis, Cancer, Breast cancer, Tumors, Tissues, Image segmentation, Pathology, Diagnostics, Imaging spectroscopy, Biopsy

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Proceedings Article | 10 May 1996 Paper

Microscopic and mesoscopic spectral bioimaging

Daniel Farkas, Byron Ballou, Gregory Fisher, Daniel Fishman, Yuval Garini, Wenhua Niu, Elliot Wachman

Proc. SPIE. 2678, Optical Diagnostics of Living Cells and Biofluids

KEYWORDS: Tumors, Tissues, Skin, Microscopy, Luminescence, Image resolution, Imaging spectroscopy, In vivo imaging, Tissue optics, Acousto-optics

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Proceedings Article | 10 May 1996 Paper

Automated interactive microscopy: measuring and manipulating the chemical and molecular dynamics of cells and tissues

D. Lansing Taylor, Lowell Harris, R. DeBiasio, Scott Fahlman, Daniel Farkas, Frederick Lanni, Michel Nederlof, Albert Gough

Proc. SPIE. 2678, Optical Diagnostics of Living Cells and Biofluids

KEYWORDS: Human-machine interfaces, Microscopes, Imaging systems, Cameras, Image processing, Microscopy, Luminescence, Image resolution, Data acquisition, 3D image processing

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Proceedings Article | 1 April 1996 Paper

Three-dimensional imaging of nucleolin trafficking in normal cells, transfectants, and heterokaryons

Byron Ballou, Gregory Fisher, Jau-Shyong Deng, Thomas Hakala, Meera Srivastava, Daniel Farkas

Proc. SPIE. 2680, Ultrasensitive Biochemical Diagnostics

KEYWORDS: Microscopes, Proteins, 3D acquisition, Tumors, Tissues, Monoclonal antibodies, Biotechnology, In vivo imaging, Systems modeling, 3D image processing

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The study of intracellular trafficking using labeled molecules has been aided by the development of the cyanine fluorochromes, which are easily coupled, very soluble, resist photobleaching, and fluoresce at far-red wavelengths where background fluorescence is minimal. We have used Cy3-, Cy5-, and Cy5.5-labeled antibodies, antigen-binding fragments, and specifically binding single-stranded oligonucleotides to follow expression and trafficking of nucleolin, the most abundant protein of the nucleolus. Nucleolin shuttles between the nucleolus and the cytoplasm, and is also expressed on the cell surface, allowing us to test our techniques at all three cellular sites. Differentially cyanine-labeled non-specific antibodies were used to control for non-specific binding. Similarly, the differentially labeled non-binding strand of the cloned oligonucleotide served as a control. The multimode microscope allowed us to follow both rapid and slow redistributions of labeled ligands in the same study. We also performed 3-D reconstructions of nucleolin distribution in cells using rapid acquisition and deconvolution. Microinjection of labeled ligands was used to follow intracellular distribution, while incubation of whole cells with antibody and antigen-binding fragments was used to study uptake. To unambiguously define trafficking, and eliminate the possibility of interference by cross-reactive proteins, we transfected mouse renal cell carcinoma cells that express cell surface nucleolin with human nucleolin. We used microinjection and cell surface staining with Cy3- or Cy5- labeled monoclonal antibody D3 (specific for human nucleolin) to assess the cellular distribution of the human protein. Several clones expressed human nucleolin on their surfaces and showed high levels of transport of the human protein into the mouse nucleus and nucleolus. This distribution roughly parallels that of mouse nucleolin as determined by labeled polyclonal antibody. We have used these engineered transfectants to determine whether the cell surface-expressed xenogeneic nucleolin can serve as a target for antibodies in vivo.

Proceedings Article | 3 April 1995 Paper

From in vitro to in vivo by dynamic multiwavelength imaging

Daniel Farkas, Byron Ballou, Gregory Fisher, D. Lansing Taylor

Proc. SPIE. 2386, Ultrasensitive Instrumentation for DNA Sequencing and Biochemical Diagnostics

KEYWORDS: Microscopes, Tumors, Tissues, Imaging systems, Microscopy, Luminescence, Monoclonal antibodies, Live cell imaging, In vivo imaging, In vitro testing

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Proceedings Article | 17 August 1994 Paper

New waves in light microscopy

Daniel Farkas, Brent Bailey, Frederick Lanni, D. Lansing Taylor

Proc. SPIE. 2137, Time-Resolved Laser Spectroscopy in Biochemistry IV

KEYWORDS: Confocal microscopy, Microscopes, 3D acquisition, Imaging systems, Sensors, Image processing, Microscopy, Luminescence, Image resolution, 3D image processing

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Proceedings Article | 4 April 1994 Paper

Three-dimensional imaging of biological specimens with standing wave fluorescence microscopy

Brent Bailey, Vijay Krishnamurthi, Daniel Farkas, D. Lansing Taylor, Frederick Lanni

Proc. SPIE. 2184, Three-Dimensional Microscopy: Image Acquisition and Processing

KEYWORDS: Microscopes, Mirrors, Modulation, Image processing, Microscopy, Luminescence, Image resolution, Refraction, Yeast, 3D image processing

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Showing 5 of 59 publications

Proceedings Volume Editor (26)

SPIE Conference Volume | 4 March 2020

Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVIII

SPIE Conference Volume | 7 June 2019

Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVII

SPIE Conference Volume | 2 May 2018

Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVI

SPIE Conference Volume | 21 April 2017

Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XV

SPIE Conference Volume | 17 June 2016

Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX

Showing 5 of 26 publications

Conference Committee Involvement (34)

Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVIII

3 February 2020 | San Francisco, California, United States

Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVII

4 February 2019 | San Francisco, California, United States

Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVI

29 January 2018 | San Francisco, California, United States

Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XV

30 January 2017 | San Francisco, California, United States

Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX

15 February 2016 | San Francisco, California, United States

Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIII

9 February 2015 | San Francisco, California, United States

Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII

3 February 2014 | San Francisco, California, United States

Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XI

2 February 2013 | San Francisco, California, United States

Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues X

21 January 2012 | San Francisco, California, United States

Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX

22 January 2011 | San Francisco, California, United States

Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII

23 January 2010 | San Francisco, California, United States

Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VII

26 January 2009 | San Jose, California, United States

Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VI

21 January 2008 | San Jose, California, United States

Advanced Biomedical and Clinical Diagnostic Systems VI

20 January 2008 | San Jose, California, United States

Confocal, Multiphoton, and Nonlinear Microscopic Imaging

17 June 2007 | Munich, Germany

Imaging, Manipulation and Analysis of Biomolecules, Cells, and Tissues V

22 January 2007 | San Jose, California, United States

Advanced Biomedical and Clinical Diagnostic Systems V

21 January 2007 | San Jose, California, United States

Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV

23 January 2006 | San Jose, California, United States

Advanced Biomedical and Clinical Diagnostic Systems IV

22 January 2006 | San Jose, California, United States

Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues II

24 January 2005 | San Jose, CA, United States

Advanced Biomedical and Clinical Diagnostic Systems III

23 January 2005 | San Jose, CA, United States

Imaging, Manipulation and Analysis of Biomolecules, Cells, and Tissues

27 January 2004 | San Jose, CA, United States

BioMEMS and Nanotechnology

10 December 2003 | Perth, Australia

Confocal, Multiphoton, and Nonlinear Microscopic Imaging

22 June 2003 | Munich, Germany

Manipulation and Analysis of Biomolecules, Cells and Tissues

28 January 2003 | San Jose, CA, United States

Advanced Biomedical and Clinical Diagnostic Systems

26 January 2003 | San Jose, CA, United States

Optical Diagnostics of Living Cells V

23 January 2002 | San Jose, CA, United States

Optical Diagnostics of Living Cells IV

24 January 2001 | San Jose, CA, United States

Functional Imaging and Optical Manipulation of Living Cells and Tissues

24 January 2000 | San Jose, CA, United States

Optical Diagnostics of Living Cells III

24 January 2000 | San Jose, CA, United States

Functional Imaging and Optical Manipulation of Living Cells

25 January 1999 | San Jose, CA, United States

Optical Diagnostics of Living Cells II

25 January 1998 | San Jose, CA, United States

Functional Imaging and Optical Manipulation of Living Cells

10 February 1997 | San Jose, CA, United States

Optical Diagnostics of Living Cells and Biofluids

31 January 1996 | San Jose, CA, United States

Showing 5 of 34 Conference Committees

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