Ultrafast pump-probe spectroscopy and pulse-shaping techniques are providing new modes of contrast for the field of
multiphoton microscopy. Endogenous species such as heme proteins show rich nonlinear spectroscopic signatures of
excited state absorption, stimulated emission and ground-state bleaching. Commercially available octave-spanning
Ti:sapphire oscillators offer new opportunities for imaging based on pump-probe contrast. Spatial light modulators take
advantage of this large bandwidth, shaping pulses of light to selectively excite molecular structures with similar spectral
properties. We present two-color pump-probe imaging of heme proteins solutions and red blood cells.