The extension of imaging flow cytometry (IFC) with snapshot-mosaic cameras is a novel approach for the in-flow characterization of bioparticles. With this innovative approach, cells at the single cell level as well as whole cell populations can be analyzed and counted. Spatial and spectrally resolving snapshot-mosaic cameras and real time capable multivariate data processing extend the possibilities for automated and in-flow characterization and analysis of mixed bioparticle populations. Cells move through a microfluidic chip for analysis and measurement as well as classification. For consistent imaging quality the cells passing an innovative 3-step microfluidic focusing unit. For the investigations we used the microalgae Haematococcus pluvialis (HP). These microalgae are used commercially to produce the red carotenoid pigment astaxanthin. Therefore, HP is suitable to practically demonstrate the usability of the developed system.
Aim of the paper is the demonstration of a paradigm shift in shape, color and spectral measurements in industry, biology and medicine as well as in measurement education and training. Innovative hardware apps (hwapps) and software apps (swapps) with smartpads are fundamental enablers for the transformation from conventional stationary working places towards innovative mobile working places with in-field measurements and point-of-care (POC) diagnostics. Mobile open online courses (MOOCs) are transforming the study habits. Practical examples for the application of innovative photonic micro shapemeters, colormeters and spectrometers will be given. The innovative approach opens so far untapped enormous markets for measurement science, engineering and training. These innovative working conditions will be fast accepted due to their convenience, reliability and affordability. A highly visible advantage of smartpads is the huge number of their distribution, their worldwide connectivity via Internet and cloud services, the standardized interfaces like USB and HDMI and the experienced capabilities of their users for practical operations, learned with their private smartpads.