We present a commercial platform for both label-free and labeled bioanalysis based on Localized Surface Plasmon
Resonance. The platform relies on mass-produced nanostructured thin films with robust and reproducible plasmon
resonances. The physical properties of these films (reproducibility, optical properties) as well as their stability, noise
level, and intrinsic detection limits will be discussed. Also, we will illustrate the performance and strength of the
platform in real-life assays. We will show how the sensitivity barrier can be lowered from the ng/mL range to the pg/mL
range using the very same chip and different implementations. The examples will demonstrate how rich, fast, simple and
reliable the platform is.
The use of quantum dots to study and solve biological issues is bringing scientists and engineers into the realm of pure biology. Yet quantum dots are still a young technology that has not fulfilled all its immense promises. The prospects of using quantum dots in biology motivates a wealth of different studies that include the targeting of organisms in live cells and the characterization of the optical properties of the bioconjugates at the single molecule level. Here, I want to review some experiments using silanized quantum dots. In particular, I want to focus on the versatility of silanized quantum dots for bioconjugation and show how they can be programmed to target the nucleus of live cells. In parallel, silanized qdots are remarkable chromophores that can be used as FRET sensors. FRET efficiencies up to 100% can be reached by tuning the spectral properties of the donor and acceptor pairs.