We have developed a line-scanned dual-axis confocal (LS-DAC) microscope with
subcellular resolution suitable for real time diagnostic imaging at shallow depths. This design
serves as a benchtop prototype for a handheld version of the LS-DAC intended for rapid point-of-care
pathology. We have assessed the performance trade-offs between the LS-DAC and a point-scanned
dual-axis confocal (PS-DAC) microscope via diffraction-theory analysis, Monte-Carlo
simulations, and characterization experiments with phantoms and fresh tissues. In addition, we are
exploring the use of a sCMOS detector array and rapid 3D deconvolution to improve the
sensitivity and resolution of our LS-DAC microscope.