In this paper we report two different methodologies for labeling ligand-gated receptors. The first of
these builds upon our earlier work with serotonin conjugated quantum dots and our studies with pegilated quantum dots
to reduce non specific binding. In this approach a pegilated derivative of muscimol was synthesized and attached via an
amide linkage to quantum dots coated in an amphiphillic polymer derivative of poly acrylamide. These conjugates were
used to image the GABAC receptor in oocytes. An alternative approach was used to image tissue sections to study
nicotinic acetylcholine receptors in the neuro muscular junction with biotinylated Bungerotoxin and streptavidin coated
quantum dots.
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