Mr. David A. C. Thomson Profile

David A. C. Thomson

at Swinburne Univ of Technology

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Proceedings Article | 29 March 2004 Paper

Protein patterning in polycarbonate microfluidic channels

David Thomson, Jason Hayes, Helmut Thissen

Proceedings Volume 5275, (2004) https://doi.org/10.1117/12.524675

KEYWORDS: Proteins, Polymers, Microfluidics, Optical lithography, Plasma, Excimer lasers, Adsorption, Coating, Laser ablation, Diffusion

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In this work protein patterning has been achieved within a polycarbonate microfluidic device. Channel structures were first coated with plasma polymerized allylamine (ALAPP) followed by the "cloud point" deposition of polyethylene oxide (PEO), a protein repellent molecule. Excimer laser micromachining was used to pattern the PEO to control protein localization. Subsequent removal of a sacrificial layer of polycarbonate resulted in the patterned polymer coating only in the channels of a simple fluidic device. Following a final diffusion bonding fabrication step the devices were filled with a buffer containing Streptavidin conjugated with fluorescein, and visualized under a confocal fluorescent microscope. This confirmed that protein adhesion occurred only in laser patterned areas. The ability to control protein adhesion in microfludic channels leads to the possibility of generating arrays of proteins or cells within polymer microfludics for cheap automated biosensors and synthesis systems.

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